Production and Characterization of Polyclonal Antibody for the N-Methylcarbamate Insecticide Metolcarb  

作　　者：ZHANG Qi LI Tie-jun ZHU Xiao-xia XU Li-na LIU Feng-quan HU Bai-shi JIANG Ying-hua CAO Bin 

机构地区：[1]Key Laboratory of Monitoring and Management of Plant Diseases and Pests, Ministry of Agriculture/Department of Plant Protection, Nanjing Agricultural University, Nanjing 210095, P.R.China [2]Pesticide Research Institute of Jiangsu Province, Nanjing 210036, P.R.China

出　　处：《Agricultural Sciences in China》2007年第2期175-179,共5页中国农业科学（英文版）

摘　　要：The hapten, 3-{ [1-（3-（methyl）phenyloxy）-carbonyl]amino}propanoic acid （HOM）, mimicking the analyte metolcarb, was synthesized and verified by mass spectrometry （MS） and 1H-nuclear magnetic resonance spectrometry （1H-NMR）. Then, HOM was conjugated with the carrier proteins bovine serum albumin （BSA） and ovalbumin （OVA） with stoichiometric amounts of N-hydroxysuccinimide/dicyclohexylcarbodimide （NHS/DCC） using the activated ester method. Polyclonal antibodies were raised against the conjugate of HOM-BSA in rabbits. Antiserum titres were determined by noncompetitive indirect ELISA procedures and the titer of pAb01 reached 1.28 × 10^6. The cross-reactivities of the structurally related Nmethylcarbamate insecticides were 0.0% except for dimethacarb. These results indicate that the antibody pAb01 with strong affinity and high specificity can be used to develop a sensitive and rapid detection protocol for metolcarb residue.The hapten, 3-{ [1-（3-（methyl）phenyloxy）-carbonyl]amino}propanoic acid （HOM）, mimicking the analyte metolcarb, was synthesized and verified by mass spectrometry （MS） and 1H-nuclear magnetic resonance spectrometry （1H-NMR）. Then, HOM was conjugated with the carrier proteins bovine serum albumin （BSA） and ovalbumin （OVA） with stoichiometric amounts of N-hydroxysuccinimide/dicyclohexylcarbodimide （NHS/DCC） using the activated ester method. Polyclonal antibodies were raised against the conjugate of HOM-BSA in rabbits. Antiserum titres were determined by noncompetitive indirect ELISA procedures and the titer of pAb01 reached 1.28 × 10^6. The cross-reactivities of the structurally related Nmethylcarbamate insecticides were 0.0% except for dimethacarb. These results indicate that the antibody pAb01 with strong affinity and high specificity can be used to develop a sensitive and rapid detection protocol for metolcarb residue.

关 键 词：metolcarb ANTIGEN polyclonal antibody antiserum characterization ELISA 

分 类 号：S482.34[农业科学—农药学]