HPLC法测定茶叶中儿茶素类及咖啡因的含量  

HPLC determination of catechins and caffeine in tea

作　　者：何昱[1] 洪筱坤[2] 王智华[2] 

机构地区：[1]浙江中医药大学,杭州310053 [2]上海中医药大学,上海201203

出　　处：《药物分析杂志》2007年第2期197-200,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立茶叶中6种儿茶素类成分(EGC、DC、EGCG、EC、GCG、ECG)和咖啡因(CAF)的 HPLC 同时定量方法,并测定23个茶叶样品中七组分的含量,为茶叶的质量评判以及茶多酚与其原料绿茶之间的关系探讨奠定基础。方法:采用 Supelco^Discovery C_(18)(250 mm×4.6 mm,5 μm)色谱柱,含0.1%甲酸的水-甲醇的梯度洗脱系统,流速0.5 mL·min^(-1),检测波长278nm。结果:在所选择的分析条件下,样品中的各组分获得了理想分离,且各组分的线性关系良好。结论:该方法准确、稳定,重复性好,可用于茶叶中七组分的定量分析。Objective： To establish a method for the simultaneous determination of catechins （ EGC, DC, EGCG, EC, GCG, ECG） and caffeine （CAF） in tea and to quantify seven ingredients in twenty -three tea samples. The obtained data can supply references to the evaluation of tea quality and the relationship discussion between tea polyphenols and their raw material. Method ：The study was achieved using a Supelco Discovery C18 column （250 mm × 4. 6 mm,5 μm） with a methanol- water- 0. 1% formic acid gradient elution system. The elution were detected at 278 nm and the flow rate was 0. 5 mL · min^-1. Results：Under the selected analytical condition, seven ingredients in the samples got ideal resolution and had good linear relationship. Conclusion：This method is accurate, stable, reproducible and suitable for the determination of catechins and CAF in tea samples.

关 键 词：茶叶 儿茶素类 咖啡因(CAF) 含量测定 

分 类 号：R917[医药卫生—药物分析学]