气相色谱-质谱联用测定莪术油中β-榄香烯的含量  

GC-MS determination of β-elemene in Zedoary turmeric oil

作　　者：杜霞[1] 吴琳华[1] 赵红光[1] 

机构地区：[1]哈尔滨医科大学附属第二临床医院药学部,哈尔滨150086

出　　处：《药物分析杂志》2007年第2期216-218,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立莪术油中β-榄香烯含量的气相色谱-质谱测定方法。方法:色谱条件为 HP-5MS 色谱柱(30 m×0.25mm×0.25μm),初始温度100℃,10℃·min^(-1)升温至220℃,载气流速1 mL·min^(-1)。溶剂延迟5 min,质谱扫描45～600amu,进样量1μL。结果:β-榄香烯浓度在0.98～9.84μg·mL^(-1)范围内呈线性关系(r=0.9990),加样回收率为98.9%(RSD=3.5%)。结论:建立的方法简单、快速,可用于β-榄香烯及其制剂的含量测定。Objective： To develop a reliable method for determination of β-elemene in Zedoary turmeric oil by gas chromatography - mass spectrometry （ GC - MS）. Method： An HP - 5 MS （ 30 m × 0. 25 mm × 0. 25 μm） column was used. Initial temperature was set at 100 ℃. It was programmed rising by 10 ℃· min^-1 until 220 ℃. Gas flow rate was set at 1 mL · min^-1. Solvent was delayed by 5 minutes. MS scanning was set at the range of 45 -600 amu. Resuits： The standard curve was linear over the range of 0. 98 - 9. 84 μg · mL^-1 （ r = 0. 9990）. The average recovery was 98.9% with the RSD of 3.5%. Conclusion：This method is simple,rapid and precise with good reproducibility. It can be used for quality assessment of β-elemene.

关 键 词：Β-榄香烯 莪术油 气相色谱-质谱联用 

分 类 号：R917[医药卫生—药物分析学]