多重耐药鸡致病性沙门氏菌I类整合子的检测研究  

Study on Detection I Integron of Multidrug Resistace Pathogenic Salmonella from Chicken

作　　者：李成忠[1] 王红宁[2] 黄勇[3] 马孟根[2] 吴琦[4] 羊云飞[3] 谢涛[3] 柳萍[3] 

机构地区：[1]四川农业大学资环学院,雅安625014 [2]四川大学"985工程"西南资源环境与灾害防治科技创新平台,成都610061 [3]四川农业大学动物科技学院,雅安625014 [4]四川农业大学生命理学院,雅安625014

出　　处：《中国畜牧兽医》2007年第1期119-123,共5页China Animal Husbandry & Veterinary Medicine

基　　金：国家"十五"重点科技攻关子课题(2002BA514A-12-5)

摘　　要：采用药敏纸片法（NCCLS2004），对19株鸡致病性沙门氏菌进行了药敏试验，结果19株菌对丁胺卡那霉素、卡那霉素、庆大霉素、新霉素、链霉素、氨苄青霉素、强力霉素、氟哌酸、头孢三嗪、氯霉素、磺胺异恶唑、四环素、先锋V13种药物的耐药率分别是：5．26％、63．16％、47．37％、36．84％、84．21％、84．2％、100％、57．9％、5．26％、52．6％、89．47％、100％、26．3％。19株菌多为多重耐药：其中耐5种、6种、7种、8种、9种、10种药物的分别为10．53％、5．26％、15．79％、21．05％、15．79％、15．79％，多重耐药率84．21％（16／19）；根据Ⅰ类整合子的结构设计了3对引物，对Ⅰ类整合子的intⅠ1、可变区、3’保守端进行扩增，检测鸡致病性沙门氏菌Ⅰ类整合子，分析Ⅰ类整合子与多重耐药的关系，扩增出了750-2000bp的基因盒片段，检出率为73．68％（14／19），多重耐药菌的Ⅰ类整合子检出率为87．5％（14／16），Ⅰ类整合子PCR阳性菌株多重耐药率为100％（14／14）。研究结果表明，Ⅰ类整合子与多重耐药之间有明显的相关性。Antimicrobial resistance test of 19 samonella isolates was done by disc dilution method（NCCLS 2003）. According to the construction of Ⅰ integron , The drug-resistance rate of amikacin, kanamycin, gentamicin, neomycin , streptomycin, ampieillin, doxyeyeline, norfloxaein, ceftriaxone, ehloramphenieol, sulfafurazole, tetracycline, cefazolin, was 5. 26%, 63. 16%, 47.37%,36.84%,84.21%,84.2%,100%,57.9%,5.26%,52.6%,89.47%,100%, 26.3% respectively. Most of the isolates were multidrug resistance and the rate was 84.21% （16/19）. Among the total of the multidrug-resistance isolates, the rate of resistance to five ,six, seven, eight ,nine and ten drugs was 10.53% ,5.26% ,15.79% ,21.05% ,15.79%, 15.79%. PCR was employed to amplify Ⅰ integron and analysis the relationship between Ⅰ integron and multidrug resistance. 3 pairs of primer was design to amplify Ⅰ integron＇s intⅠ1 gene, variable segment and 3＇-conserved segment. As a result, we amplified several fragments with different length from 750 to 2000 bp. Among these 19 isolates, the detection rate of Ⅰ integron was 73.68% （14/ 19）, and among these 16 isolates which had been proven multidrug-resistant,the detection rate of Ⅰ integron was 87.5%. The Ⅰ integron positive isolates＂ rate of multidrug-resistanee was 100 % （14/14） . The results shows Ⅰ integron obviously has the relationship with multidrug resistance.

关 键 词：沙门氏菌 Ⅰ类整合子 多重耐药 检测 

分 类 号：S852.612[农业科学—基础兽医学]