菌核净残留免疫分析半抗原的制备和鉴定  

Synthesis and Identification of the Hapten N-(3,5-Dichlorophenyl) Succinimide-2-Hemisuccinate for Immunoassay

作　　者：刘蓉蓉[1] 刘贤进[1] 刘媛[2] 方敦煌[3] 胡秋辉[1] 

机构地区：[1]南京农业大学食品科技学院,江苏南京210095 [2]江苏省农业科学院农药生物生态实验室,江苏南京210014 [3]云南省烟草科学研究所,云南玉溪653100

出　　处：《食品科学》2007年第2期223-226,共4页Food Science

基　　金：863计划(2006AA068411);云南省烟草公司(05-6)

摘　　要：菌核净属于半抗原生物小分子,具有免疫反应性,但不具有免疫原性。为了建立菌核净农药残留的免疫分析方法,以苹果酸氯醛交酯和3,5-二氯苯胺为原料,合成N-(3,5-二氯苯基)-2-羟基琥珀酰亚胺,然后与琥珀酸酐反应,合成了菌核净半抗原N-(3,5-二氯苯基)琥珀酰亚胺-2-琥珀酸半酯,产物经红外、核磁和液质连用等方法确认,为研究菌核净的免疫分析奠定基础。Dimethachlon is a kind of small molecular hapten that has immunoreactivity instead of immunogenicity. In order to establish an immunoassay method for dimethachlon, N-（3,5-dichlorophenyl）-2-hydroxysuccinimide was synthesized by chloralide of malic acid and 3,5-dichloroaniline, and then reacted with succinic anhydride to obtain the hapten N-（3,5- dichlorophenyl） succinimide-2-hemisuccinate. The structure of the product was identified by IR, IHMR and LC-MS, which provided the foundation for future research of dimethachlon immunoassay.

关 键 词：菌核净 半抗原 合成 

分 类 号：S482.26[农业科学—农药学]