用改进的超声波-溶菌酶法制备钝顶螺旋藻原生质球  

Preparation of spheroplasts from Spirulina platensis by ultrasonic-lysozyme method

作　　者：银红梅[1] 张学成[1] 王志刚[1] 

机构地区：[1]中国海洋大学生命科学与技术学部,山东青岛266003

出　　处：《海洋科学》2007年第2期44-47,共4页Marine Sciences

摘　　要：用改进的超声波-溶菌酶法制备钝项螺旋藻原生质球。钝项螺旋藻（Spirulina platensis）经超声波破碎后，用1.5mol／L NaCl的Zarrouk培养基洗去细胞外壁的胶质鞘，然后进行酶解。酶解条件为：pH7．2的0.2mol／L磷酸钾缓冲液，0．8mol／L KCl，0．5%溶菌酶，28-30℃水浴震荡酶解5～7h，获得40％以上有活力的钝项螺旋藻原生质球。The obtainment of spheroplasts of Spirulina platensis by the improved ultrasonie-lysozyme method was dealt with in the paper. S. platensis was broken by ultrasonic, and the gelatinous sheath of the cells was rinsed with Zarrouk medium containing 1.5 mol/L NaCl. Then the cells were digested by lysozyme at 28-30℃ for 5-7 hours, the enzyme solution was composed of 0. 8 mol/L KCl, 0. 5% lysozyme and 7.2 pH, 0.2 mol/L buffer of K2HPO4 and KH2PO4. Results showed that spheroplasts rate could be obtained above 40%.

关 键 词：钝顶螺旋藻(Spirulina platensis) 原生质球 超声波 溶菌酶 

分 类 号：TS201.21[轻工技术与工程—食品科学]