基质固相分散-高效液相色谱法同时测定烟草中的有机酸  

Determination of six organic acids in tobacco by high-performance liquid chromatography based on matrix solid phase dispersion

作　　者：陈章玉[1] 缪明明[1] 孔维松[1] 杨光宇[1] 苏庆德[2] 

机构地区：[1]云南烟草科学研究院化学室 [2]中国科技大学化学系,合肥230026

出　　处：《中国烟草学报》2007年第1期11-14,共4页Acta Tabacaria Sinica

基　　金：云南省烟草工业公司重点资助项目(2004B205)

摘　　要：研究了用基质固相分散-高效液相色谱法测定烟草中有机酸的方法。将烟草样品与石墨化碳黑球(W/W,1∶4)充分研磨后装柱,用热水洗脱,洗脱液中的有机酸用高效液相色谱测定。以ZORBAX Stable Bound(4.6×50mm,1.8μm)快速分离柱为固定相,0.01mol/LNaH2PO4(pH2.98)和乙腈(体积比为98:2)为流动相,流速为2.0mL/min,柱温25℃,检测波长210nm。方法回收率为94%～98%;相对标准偏差为2.1%～3.0%;检出限为80～200μg/L。色谱分离时间不超过3.0min。用于实际样品分析,结果令人满意。A new method for the determination of organic acids in tobacco based on matrix solid phase dispersion was established. Samples and graphitized carbon black sorbent （ 1 ： 4） were homogenized in a mortar for 5 min and then packed into a cartridge. Organic acids were eluted from the cartridge with water and then analyzed by high-performance liquid chromatography with 2% methanol （containing 0.01 mol/L NaH2PO4 buffer, pH 2.98） as mobile phase at a flow rate of 2.0 mL/min and detected at wavelength of 210 nm. The 6 organic acids were separated on a ZORBAX Stable Bound （4.6 x 50 mm, 1.8μm） column within 3 min. Limits of detections （S/N = 3） were below 200 μg/L. Mean recoveries ranged from 94% to 98%, and relative standard deviations were below 3.0 %. This method has been successfully applied to the determination of organic acids in tobacco with satisfactory results.

关 键 词：高效液相色谱法 有机酸 烟草 基质固相分散 

分 类 号：TS411[农业科学—烟草工业]