细胞化学染色在急性白血病分型中的应用  

Application of cytochemistry staining in classification of acute leukemia

作　　者：宫继勇[1] 毕早斌 田万林[1] 

机构地区：[1]蚌埠医学院附属医院检验科,安徽蚌埠233000 [2]安徽省池州市公安局刑警支队,247100

出　　处：《蚌埠医学院学报》2007年第2期212-213,共2页Journal of Bengbu Medical College

摘　　要：目的:探讨细胞化学染色法对急性白血病(acute leukemia,AL)诊断分型的价值。方法:骨髓涂片常规瑞氏染色,细胞化学染色包括过氧化物酶(POX)、特异性酯酶(CE)、非特异性酯酶(NAE)(即α-醋酸萘酚酯)、糖原(PAS)(即过碘酸雪夫)染色。结果:POX、CE、PAS可区别急性淋巴细胞白血病(ALL)与急性非淋巴细胞白血病(ANLL),NAE加氟化钠抑制试验对区分粒系和单核系白血病有重要意义。细胞形态学对AL的确诊率为74.69%,加做细胞化学染色可将诊断率提高到90.1%。结论:细胞化学染色对AL的确诊分型和鉴别诊断是不可缺少的客观指标,可以明显提高AL的诊断率,且方法稳定,操作简便。Objective：To diagnose and classify acute leukemia （AL）by cytochemistry staining. Methods：Bone marrow slides were stained with wright staining and cytochemistry staining including peroxidase （POX）, naphthol AS-D chloroacetate esterase （CE）, and alpha naphthyl-acetate esterase （NAE）with sodium fluoride inhibition and periodic acid Schiff reaction （PAS）. Results：POX,CE and PAS could distinguish acute nonlymphocytic leukemia （ANLL） from acute lymphocytic leukemia （ALL）. NAE with sodium fluoride inhibition could differentiate granulocytic leukemia from monocytic leukemia. Furthermore, the diagnostic rate of AL increased from 74.4% with only morphology to 90.1% with both cytochemistry and morphology. Conclusions： Cytochemistry staining is of great value for the diagnosis and classification of AL and can increase the conformity rate.

关 键 词：白血病 细胞技术 细胞化学染色 分型 

分 类 号：R733.71[医药卫生—肿瘤] R446.13[医药卫生—临床医学]