抗肿瘤坏死因子单克隆抗体对Bcl-2基因蛋白及mRNA表达的影响  

The effect of anti-TNF-mAb on the protein and mRNA expression of bcl-2 gene

作　　者：贾清华[1] 张万义[1] 李小鹰[2] 

机构地区：[1]胜利石油管理局胜利医院,东营257055 [2]解放军总医院

出　　处：《陕西医学杂志》2007年第3期282-285,共4页Shaanxi Medical Journal

摘　　要：目的:探讨抗肿瘤坏死因子单克隆抗体对持续缺血和缺血再灌注损伤时心肌细胞Bcl-2基因蛋白及mRNA表达的影响。方法:大白鼠随机分为7组,以活结结扎左冠状动脉的前降支,分别造成阻断冠脉血流和再灌注。以S-P免疫组化及逆转录聚合酶链反应(RT-PCR)法分别检测Bcl-2基因蛋白与mRNA的表达变化。结果:抗肿瘤坏死因子单克隆抗体处理组与持续缺血组、缺血再灌注组比较Bcl-2基因蛋白及mRNA表达明显增强。结论:抗肿瘤坏死因子单克隆抗体预处理能上调Bcl-2基因蛋白及mRNA表达。Objective, To study the effect of anti-TNF-mAb on the expression of protein and mRNA of Bcl-2 gene in the myocardial cells during continuous ischemia and ischemia reperfusion injury. Methods： Wistar rats were randomly divided into 7 groups. The anterior descending branches of the left coronary artery was ligated with slipknot to block the blood flow and the myocardium was reperfused. Changes of the expression of protein and mRNA of Bcl-2 gene were examined by S-P immunohistochemical staining and RT-PCR analysis respectively. Results： Compared with continuous ischemia group and ischemia reperfusion group, the expression of protein and mRNA of the Bcl-2 geno was increased significantly in the anti-TNF-mAb-administrated group. Conclusion： Pretreatment with anti-TNF-mAb could up-regulate the expression of protein and mRNA of Bcl-2 gene.

关 键 词：心肌再灌注损伤/免疫学Bel-2基因/免疫学肿瘤坏死因子α/免疫学 抗体 单克隆/免疫学 mRNA 兔 

分 类 号：R739.5[医药卫生—肿瘤]