Dynamic change of protein polypeptide of Ginkgo biloba seed during germination  

作　　者：GUO Hong-yan LI Sheng-ping PENG Fang-ren 

机构地区：[1]College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, P. R.. China

出　　处：《Journal of Forestry Research》2007年第1期35-38,共4页林业研究（英文版）

基　　金：The research was supported by National Natural Science Foundation of China (30371154).

摘　　要：The dynamic changes of protein polypeptide in endosperms of Gingkgo biloba seeds during seed germination were studied by SDS-PAGE and two-dimensional gel electrophoresis （2-DE）. The results showed that 80 kinds of protein spots in endosperms of Gingkgo biloba were clear observed in the 2-DE spectrum. Protein molecular weights were in the range of 26-52kD, and their isoelectric points were in the range of 5.8-7.8. In the course of seed germination, 13 kinds of proteins were degraded, and 13 kinds of proteins were synthesized; 7 kinds of proteins with different molecular weights and isoelectric points of 35kD/pIr.8, 31kD/pIr.8, 29kD/pIr.8, 33kD/pI6.6, 33kD/pI 6.4, 34kD/pI7.7 and 31kD/pI7.7 were identified primarily as vegetative storage proteins （VSPs）.The dynamic changes of protein polypeptide in endosperms of Gingkgo biloba seeds during seed germination were studied by SDS-PAGE and two-dimensional gel electrophoresis (2-DE). The results showed that 80 kinds of protein spots in endosperms of Gingkgo biloba were clear observed in the 2-DE spectrum. Protein molecular weights were in the range of 26-52kD, and their isoelectric points were in the range of 5.8-7.8. In the course of seed germination, 13 kinds of proteins were degraded, and 13 kinds of proteins were synthesized; 7 kinds of proteins with different molecular weights and isoelectric points of 35kD/pI6.8, 31kD/pI6.8, 29kD/pI6.8, 33kD/pI6.6, 33kD/pI 6.4, 34kD/ pI7.7 and 31kD/pI7.7 were identified primarily as vegetative storage proteins (VSPs).

关 键 词：Ginkgo biloba seed GERMINATION PROTEIN ELECTROPHORESIS 

分 类 号：S792.95[农业科学—林木遗传育种]