Effect of β-TCP Ceramic on the Total Protein of Osteoblasts  

作　　者：齐志涛 

机构地区：[1]The Bio-material Research and Engineering Center, Wuhan University of Technology

出　　处：《Journal of Wuhan University of Technology(Materials Science)》2007年第1期98-101,共4页武汉理工大学学报（材料科学英文版）

基　　金：Funded by the "973" Chinese National Key Fundamental Research and Development Program (No.G1999064701);Key Technologies R&D Program of Hubei Province (No.2004AA303B03)

摘　　要：The osteoblast with the β-TCP ceramic was co-cultured. Scanning electron microscopy shows the cells adhered onto β-TCP ceramic surface and grew better, proving the β-TCP ceramic has a biocompatible property. The Coomassie brilliant blue G-250 stain shows that the total protein in the experimental groups was highly increased compared with the control group （p〈0.05）. Flow-cytometric analysis shows that β-TCP ceramic could promote the osteoblast transform from the G0/G1 phase to S phase. Those all demonstrate the product of the degradation of the β-TCP ceramic may take part in the protein metabolism of osteoblast.The osteoblast with the β-TCP ceramic was co-cultured. Scanning electron microscopy shows the cells adhered onto β-TCP ceramic surface and grew better, proving the β-TCP ceramic has a biocompatible property. The Coomassie brilliant blue G-250 stain shows that the total protein in the experimental groups was highly increased compared with the control group （p〈0.05）. Flow-cytometric analysis shows that β-TCP ceramic could promote the osteoblast transform from the G0/G1 phase to S phase. Those all demonstrate the product of the degradation of the β-TCP ceramic may take part in the protein metabolism of osteoblast.

关 键 词：β-TCP ceramic OSTEOBLAST flow-cytometric analysis 

分 类 号：R318.08[医药卫生—生物医学工程]