原花青素抗晶状体氧化损伤的实验研究  

The experimental study on the prevention of cataract with procyanidins

作　　者：张俊鸽[1] 李平华[1] 

机构地区：[1]重庆医科大学附属第一医院眼科,重庆400016

出　　处：《重庆医科大学学报》2007年第4期387-391,共5页Journal of Chongqing Medical University

摘　　要：目的:观察原花青素(Procyanidins,PC)对氧化损伤晶状体的形态学及抗氧化系统的影响。方法:将正常兔晶状体分为4组:Feton模型组(氧化损伤组)、维生素C组(100mg/L)、维生素E组(100mg/L)、PC组(50mg/L、100mg/L、200mg/L)。体外培养24h后,观察晶状体混浊情况,分析晶状体的相对灰度值,光镜下观察组织形态学变化。检测各组晶状体可溶性蛋白(slo-Prot)含量,抗氧化酶系统中的超氧化物歧化酶(SOD)活性以及脂质过氧化反应终产物丙二醛(MDA)的含量。结果:各组晶状体有不同程度混浊,Feton组和VC组较其它组相对灰度值减小(P<0.05),PC组晶状体组织结构较其它组损伤轻。和其它组相比,PC组slo-Prot含量增加,SOD活性增高,MDA的含量降低(P<0.01)。结论:PC可提高氧化损伤晶状体的抗氧化能力,降低脂质过氧化物水平,减小晶状体损伤程度。Objective：To evaluate the effect of procyanidins in protecting lens against oxidative stress.Methods：Rabbit＇s lenses cultured in vitro were divided into 4 groups：Feton model group（oxidative stress group）,groups treated with 100mg/L vitamin C, 100mg/L vitamin E, and with PC（50mg/L,100mg/L,200mg/L）.Afier 24 hours of incubation,alteration of opacity was observed by photography and picture analysis;the histomorphology of lenses were examined under light microscope. The levels of slo-Prot, SOD,and MDA were tested.Resets：The transparency of all lenses were different.The relative gray scale in Feton model and vitamin C group were lower than that in other groups（P〈0.05）. Compared with other groups ,the oxidative damage of lenses in PC group was light,but slo-Prot levels and SOD activities increased,while MDA level decreased significantly （P〈0.01）. Conclusion：PC can protect lens against oxidative stress and inhibit lipid peroxidation activity.

关 键 词：原花青素 抗氧化 自由基 晶状体 保护 

分 类 号：R776.1[医药卫生—眼科]