宫颈癌组织中survivin和CD44v6的表达及其与HPV_(16/18)感染的相关性  

Expression of survivin and CD44v6 in uterine cervical carcinoma and its association with HPV_(16/18) infection

作　　者：赵富玺[1] 穆雅琴[1] 郭俊成[1] 刘润花[2] 

机构地区：[1]大同大学医学院免疫学与病原生物学研究所,山西大同037008 [2]大同大学医学院外科实验室,山西大同037008

出　　处：《基础医学与临床》2007年第3期288-292,共5页Basic and Clinical Medicine

基　　金：山西省科技厅自然基金(20051115)

摘　　要：目的研究survivin、CD44v6在宫颈癌组织中的表达及其与HPV16/18感染的相关性,以探讨宫颈癌的发生机制。方法用免疫组化方法进行survivin和CD44v6的检测,用PCR检测HPV16/18感染情况。结果survivin和CD44v6的阳性率在宫颈癌组织中远高于CIN和正常宫颈组织,差异显著(均P<0.01),其表达率与淋巴结转移有关(均P<0.05)。CD44v6随着临床分期、病例分级的升高阳性率升高(P<0.05),survivin的阳性率则随着病例分级的升高而增加(P<0.05)。HPV16/18在正常宫颈组织、CIN和宫颈癌中的阳性率逐渐升高(P<0.01),但与临床分期、病理分级、淋巴转移无关。survivin与HPV16/18感染有相关性(P<0.05)。结论宫颈癌组织中survivin的异常表达与HPV16/18感染有关。survivin和CD44v6与宫颈癌的恶变程度有关,可作为宫颈癌筛查预后判断的有利指标。Objective To investigate the relationship between expression of survivin and CD44v6 and HPV16/18 infection in uterine cervical carcinogenesis. Methods Using Streptavidin-Peroxidase （S-P） immunohistochemical technique, the authors examined the expression of survivin and CD44v6 in samples. The infection of HPV type 16, 18 DNA was determined by PCR. Results There were significant differences for survivin and CD44v6 between carcinomas, CIN and normal cervices （P 〈 0. 01, respectively）. Expression of CD44v6 was correlated with tumor gradings, clinical stages and lymph node metastasis （ P 〈 0. 05 ） , and expression of survivin was associated with tumor grades and lymph node metastasis（ P 〈0. 05 ）. The positive rate of HPV16/18 was significant different among cervical carcinomas, CIN and normal tissues（P 〈0. 01 ）but was not associated with tumor grading, clinical stages and lymph node metastasis. The infection of HPV16/18 was associated with the expression of survivin（ P 〈 0. 05 ）. Conclusion The abnormal expression of survivinis associated with the infection of HPV16/18. survivin and CD44v6 possibly become useful indexes for the primary screening and prognosis of cervical carcinomas.

关 键 词：SURVIVIN CD44V6 人乳头瘤病毒(HPV) 宫颈癌 

分 类 号：R737.33[医药卫生—肿瘤]