内皮素-1及其受体拮抗剂对HSC-T6细胞Ⅰ型胶原mRNA表达的作用  

Effect of Endothelin-1 and Its Receptor Antagonists on the Expression of Collagen-1 mRNA in HSC-T6 Cells

作　　者：张军[1] 张忠涛[1] 王宇[1] 王萍[2] 李建设[1] 周延忠[1] 

机构地区：[1]首都医科大学附属北京友谊医院普通外科 [2]首都医科大学附属北京友谊医院病理生理学实验室

出　　处：《首都医科大学学报》2007年第1期68-70,共3页Journal of Capital Medical University

基　　金：北京市卫生局重点学科(1998卫科扶字11)资助项目

摘　　要：目的检测与探讨内皮素-1及其受体拮抗剂对肝星状细胞(HSC-T6)细胞Ⅰ型胶原mRNA表达的作用和影响机制。方法采用反转录聚合酶链反应法(reverse transcription-polymerase chain reaction,RT-PCR),进行半定量分析。结果与空白对照组相比,ET-1组和ET-1+BQ-123组Ⅰ型胶原mRNA的表达显著升高(0.706±0.027、0.724±0.059比0.400±0.047,P<0.05);与ET-1组相比,ET-1+BQ-788组和ET-1+BQ-123+BQ-788组Ⅰ型胶原mRNA的表达显著降低(0.427±0.131、0.382±0.022比0.706±0.027,P<0.05)。结论ET-1使HSC-T6细胞Ⅰ型胶原mRNA的表达明显升高,选择性ETB受体拮抗剂BQ-788能抑制ET-1引起的Ⅰ型胶原mRNA的高表达,而选择性ETA受体拮抗剂BQ-123则对其无明显作用。Objective To study the effects of endothelin-1 and its receptor antagonists on the expression of collagen-1 mRNA in HSC-T6 cells. Methods RT-PCR analysis was used in the research. Results The expression of collagen-1 mRNA in ET-1 and ET-1 ＋ BQ-123 group was significantly higher than that of control group （0. 706 ± 0. 027 and 0.724 ± 0.059 vs 0. 400 ± 0. 047, P 〈 0.05 ）. The expression in ET-1 ＋ BQ-788 group and ET-1 ＋ BQ-123 ＋ BQ-788 group was significantly lower than ET-1 group （0. 427 ±0. 131 and 0.382 ±0.022 vs 0. 706 ±0.027, P〈0.05）. Conclusion ET-1 can induce high expression of collagen-1 mRNA in HSC. BQ-788, and the selective ETB receptor antagonist, can inhibit high expression of collagen-1 mRNA in HSC-T6 induced by ET-1.

关 键 词：内皮素-1 肝星状细胞 Ⅰ型胶原 反转录多聚酶链反应 

分 类 号：R575.2[医药卫生—消化系统]