人巨细胞病毒UL132基因在先天感染患儿临床株中的变异研究  

Genetic variability of human cytomegalovirus UL132 gene in strains from congenitally infected infants

作　　者：孙峥嵘[1] 吉耀华[1] 阮强[1] 何蓉[1] 齐莹[1] 马艳萍[1] 毛志芹[1] 黄郁晶[1] 王岳平[1] 

机构地区：[1]中国医科大学附属第二医院病毒研究室,沈阳110004

出　　处：《中华微生物学和免疫学杂志》2007年第2期145-150,共6页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金资助项目（30371492）

摘　　要：目的 研究人巨细胞病毒（human cytomegalovirus，HCMV）UL132基因序列在先天性感染患儿中的多态性，探讨HCMV基因多态性与其感染引起不同临床症状的关系。方法 对30株经荧光定量PCR方法（Q-PCR）检测HCMV DNA为阳性的临床株进行HCMVUL132全序列PCR扩增，并对PCR扩增产物进行序列测定及分析。结果 30株HCMV临床株的测序结果与HCMV Toledo株进行序列比较分析显示，临床株UL132开放阅读框架（open reading frame，ORF）间存在着高度的多态性，基因变异主要集中在UL132ORF的5’端。30株HCMV临床株种系进化树分析结果显示，UL132序列可分为3个基因型，黄疸患儿分布以G1型为主；神经损伤患儿以G2型为主；巨结肠患儿分别见于G1、G2、G33个基因型。所有临床株的UL132编码蛋白是疏水性蛋白，含有信号肽及跨膜蛋白区域，并在信号肽和跨膜蛋白区域之间存在2个保守的N-糖基化位点。结论 HCMVUL132基因在临床株中存在着高度的多态性。来自不同临床症状的HCMVUL132基因及其编码蛋白具有一定的结构特点，提示UL132基因及其所编码的蛋白在HCMV的致病性上可能起重要作用。Objective To investigate the polymorphism of human cytomegalovims UL132 gene in clinical strains and to find the relationship between the polymorphism and different pathogenesis of congenital HCMV infection. Methods Thirty clinical strains, which had been proven containing detectable HCMV DNA by using FQ- PCR, were amplified by PCR for full sequences of HCMV UL132. PCR amplification products were sequenced directly and the sequence data were analyzed. Results The results showed that a large number of nucleotide nonsynonymous substitutions occurred in the UL132 ORF, particularly in the 5＇ half, in comparison to the UL132 of reference strain Toledo. The UL132 variants of the clinical strains were divided into three major groups in phylogenetic tree： G1（10/30）, G2（9/30）, and G3（11/30）. It was found that the predicted UL132 products of studied strains contained hydrophobic domain. Two potential consensus sequences of N-linked carbohydrates （NMT34 and NMT63） were found between the predicted signal sequence （amino acids 1 to 24） and the transmembrane anchor （ amino acids 84 to 106）. Conclusion The precise definition of UL132 genotypes and their putative functions may facilitate understanding of the HCMV.

关 键 词：人巨细胞病毒 UL132基因 基因多态性 

分 类 号：R686[医药卫生—骨科学]