反相高效液相色谱／傅立叶变换红外光谱同轴流动雾化接口  

A New Reversed phase HPLC/FTIR Interface with a Concentric Flow Nebulizer

作　　者：许亚非[1] 

机构地区：[1]太原师专生化系

出　　处：《分析测试仪器通讯》1996年第4期202-207,共6页

摘　　要：介绍了适用于反相高效液相色谱／傅立叶变换红外光谱（ＨＰＬＣ／ＦＴＩＲ）联用的同轴流动雾化型接口（ＣＦＮ），可在５０μＬ／ｍｉｎ流速下迅速去除１００％水相，并保持较好的色谱分离度。应用ＣＦＮ技术，能显著减小ＬＣ组分的析出面积（约００１ｍｍ２），在挥发性缓冲剂存在下，其检测限低于１ｎｇ。对实时和非实时ＣＦＮ接口的仪器原理、特点及应用进行了简要阐述。A new reversed_phase HPLC/FTIR interface with a concentric flow nebulizer is introduced. By this interface up to 100% water in a mobile phase can be efficiently removed at flow rate of 50 μL/min without deteriorating effect on chromatographic resolution. Each component eluted from HPLC can be deposited in an area of 0.01 mm 2. A detection limit of less than 1 ng is possible for on line measurements even when volatile buffers are used. The principle, analytical features and applications for the instrument with on line and off line interfaces are also discussed.

关 键 词：接口 同轴流动雾化 液相色谱 红外光谱 分析化学 

分 类 号：O657.72[理学—分析化学]