野木瓜皂甙对HL60肿瘤细胞增殖的影响  

Effects of Stauntoniae Saponin on proliferation of cancer cell HL60

作　　者：叶文博[1] 李兴玉[1] 吴宗袆 韩宁宁[1] 

机构地区：[1]上海师范大学 生命与环境科学学院,上海200234

出　　处：《上海师范大学学报（自然科学版）》2007年第1期65-68,共4页Journal of Shanghai Normal University(Natural Sciences)

摘　　要：研究野木瓜皂甙(Stauntoniae Saponin,SS)提取物对人类白血病细胞系HL60增殖的影响,为中草药抗肿瘤提供实验依据．以不同浓度SS作用于HL60细胞,然后用MTT比色法测定它对HL602细胞增殖的影响．当浓度为18mg/L时,SS能明显地促进HL60细胞的增殖(P＜0．01);浓度大于90 mg/L时,SS对HL60细胞增殖产生抑制作用;当浓度为2253mg/L时,抑制率高达63．15%(P＜0．01)．浓度大于18mg/L后,细胞增殖的抑制率与SS浓度呈线性相关,r=0．8928(P＜0．001);SS的LC50为1652 mg/L．野木瓜皂甙提取物对HL60细胞的增殖有明显的双向调节作用,即在较小范围的低浓度区域内有促进HL60细胞增殖的作用,浓度增大后则改变为抑制作用;浓度越大,抑制作用越强．To investigate the effects of Stauntoniae Saponin （SS） on the proliferation of Human acute promyelocytic leukemia cell line HL60. Methods： After treated with SS with different concentrations, the proliferation of HL60 were tested by MTr assay. Results： As in 18mg/L, SS could promote the proliferation of HL60 （ P 〈 0. 01 ）. With its increasing concentration （ 0.090mg/mL）, SS reversed its effect on HL60 significantly. The ratio of inhibition on proliferation of HL60 was up to 63.15% when the concentration was in 2253mg/L （ P 〈 0. 01 ）. The ratio of inhibition was linear relation to the concentration of SS when concentration of SS was greater than 18mg/L r = 0. 8929 （P 〈 0. 001 ）. And its LC50 was 1652 mg/L. Conclusion： SS has bidirectional regulating effects on the proliferation of HL60. With the increasing concentration, the inhibiting effect of SS on HL60 would be greater.

关 键 词：野木瓜皂甙 HL60细胞 MTT比色法 双向调节 IC50 

分 类 号：Q279[生物学—细胞生物学] R362[医药卫生—病理学]