胃舒散对消炎痛所致大鼠小肠损伤的保护作用  

Protective Effect of Weishusan on Small Intestinal Lesions Induced by Indomethacin in Rats

作　　者：吴灵飞[1] 冯家琳[1] 李国平 蒲泽锦[1] 苏剑东[1] 

机构地区：[1]汕头大学医学院第二附属医院消化内科,广东汕头515041

出　　处：《汕头大学医学院学报》2007年第1期24-27,F0003,共5页Journal of Shantou University Medical College

基　　金：广东省中医药管理局资助项目(103066)

摘　　要：目的:研究胃舒散对消炎痛所致大鼠小肠损伤的保护作用及机制。方法:皮下注射消炎痛(10 mg/kg)制作大鼠小肠损伤模型。用光镜、扫描电镜和透射电镜观察不同剂量[3.0、1.5、0.75 g/(kg.d)]胃舒散对小肠损伤的影响,同时检测小肠黏膜髓过氧化物酶(MPO)、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量以及血清NO含量。结果:消炎痛引起明显的小肠黏膜损伤,伴有NO、MPO、MDA含量增加及SOD活性下降(P<0.05,P<0.01)。胃舒散剂量依赖性地减少小肠黏膜损伤指数,提高了胃组织SOD活性(P<0.05)、降低了MPO、MDA含量(P<0.05),但对血清NO水平无明显影响(P>0.05)。结论:氧自由基及NO参与了消炎痛所致小肠黏膜的损伤,胃舒散对此病变有明显保护作用,其机制与抗氧化作用有关。Objective： To study the protective effect of Weishusan on small intestinal lesions induced by indomethacin in rats. Methods： Wistar rats were injected indomethacin（suspension 10 mg/kg）subeutaneously, and the small intestine was examined for lesions 24 hours later. The different doses[3.0, 1.5, 0.75 g/（ kg·d） ] of Weishusan were administered i.g. twice, intestinal mucosal changes were observed by quantitative histology, scanning electron microscopy and transmisson electron microscopy. Superoxide dismutase （ SOD ）, myeloperoxidase（MPO） activities and malondialdehyde （MDA）levels in intestinal tissues and nitric oxide（NO）levels in senan were determined. Results： Severe lesions in the jejunum and ileum were caused by indomethacin and the NO, MPO and MDA contents were markedly increased（ P 〈 0.05）. Weishusan dose-dependently prevented the intestinal lesions（ P 〈 0.05）, enhanced SOD activities（ P 〈 0.05） and decreased MPO and MDA levels（P 〈 0.05） but not affected NO levels（P 〉 0.05）. Conclusion： Oxygen free radicals and NO may play important roles in intestinal lesions induced by indomethacin. Weishusan can protect the small intestine by antioxidation mechanism.

关 键 词：小肠损伤 消炎痛 胃舒散 一氧化氮 超氧化物歧化酶 丙二醛 

分 类 号：R573.3[医药卫生—消化系统]