丁酸钠对结肠癌细胞凋亡活性的影响  

Effect of sodium butyrate with different density on apoptosis of colorectal cancer cells

作　　者：张孝卫[1] 刘成霞[2] 王兵[1] 李铁军[1] 黄丽华[1] 王丽珍[1] 张尚忠[2] 

机构地区：[1]济南市医学科学研究所,山东济南250013 [2]山东大学齐鲁医院,山东济南250012

出　　处：《现代肿瘤医学》2007年第3期313-315,共3页Journal of Modern Oncology

基　　金：济南市计划内课题(编号:济科计2003-12-31)

摘　　要：目的观察丁酸钠对结肠癌细胞凋亡活性的影响。方法采用流式细胞术(FCM)和DNA末端原位标记染色法(TUNEL)检测细胞凋亡。结果实验48小时检测,对照组和2.5mM、5mM和10mM各浓度丁酸钠作用后的Lovo细胞凋亡率分别为11.71%、21.62%、34.40%和47.83%,各组之间有显著性差异(P<0.01);对照组和5mM丁酸钠组的HT-29细胞,培养24、48、72小时,其凋亡率分别为6.22%、12.00%、14.31%和30.68%、34.60%、41.88%,差异有显著性(P<0.01);本研究中用二倍体的人胚肾293细胞作了对照研究,未发现丁酸钠对293细胞的生长抑制和凋亡有影响。结论一定浓度的丁酸钠可以选择性抑制两种结肠癌细胞增生,诱导凋亡,显示出浓度、时间依赖性。在预防肿瘤发生的过程中发挥重要的作用。Objective： To observe the effect of sodium but）rate on apoptosis of colorectal cancer cells. Methods： TUNEL staining and FCM were used to detect the effects on cell apoptosis. Results ：The apoptotic rates （21.62%, 34.40% ,47.83 % ） of Lovo cells increase along with the density of sodium butyrate （ 2.5mM, 5 mM , 10mM ） , much higher than the control（ 11.1% ） ;The apoptotic rates （30.68% ,34.60% and 41.88% ）of HT29 cells at 5mM sodium butyrate increase at different cultivating time （24h ,48h ,72h ）, much higher than the control（ 6.22%, 12.00%, 14.31% ） ;There was no effect of sodium butyrate on proliferation and apoptosis of 293 cell. Conclusion： Sodium butyrate can inhibit the proliferation of colorectal cancer cells and induce their apoptosis selectively, and this effect is time - density - dependent.

关 键 词：丁酸盐 抗肿瘤 凋亡 HT-29 Lovo流式细胞术 

分 类 号：R730.53[医药卫生—肿瘤]