WT1在上皮性卵巢癌中的表达及意义  

Expression and clinical significance of WT1 in epithelial ovarian cancer

作　　者：郭立丽[1] 刘少扬[1] 江大琼[1] 

机构地区：[1]武汉大学中南医院妇瘤科,湖北武汉430071

出　　处：《现代肿瘤医学》2007年第3期398-401,共4页Journal of Modern Oncology

摘　　要：目的探讨肾母细胞瘤基因(Wilms tumor gene,WT1)蛋白在上皮性卵巢癌中的表达及临床意义。方法采用免疫组织化学SP法检测50例卵巢上皮性癌、10例卵巢良性肿瘤和10例正常卵巢石蜡标本中WT1表达情况。应用SPSS13.0软件结合临床病理资料进行综合分析。结果正常及良恶性上皮性卵巢组织中WT1皆有表达,WT1在卵巢癌组织中的表达明显高于正常及良性卵巢组织(P<0.01),正常和良性卵巢组织中两者无显著性差异(P>0.05)。WT1在浆液性卵巢癌中表达率为93%(28/30),随着病理分级及临床分期的增高,WT1表达率逐渐增高。WT1阴性表达者生存时间明显比WT1阳性者长。WT1表达与病理分级、临床分期、组织学类型及预后明显相关(P<0.05),而与年龄、淋巴结转移无关(P>0.05)。结论WT1在卵巢癌组织中的表达明显高于正常及良性卵巢组织,在卵巢癌的浸润和转移中发挥了重要作用,可作为判断卵巢癌恶性程度及评估不良预后的重要指标。Objective： To investigate the expression of Wilms tumor gene（ WT1 ） protein in human epithelial ovarian cancer and its clinical significance. Methods： The expression of WT1 proteins were examined in 50 cases of epithelial ovarian cancer（EOC） , 10 cases of benign epithelial tumor（BET） , 10 cases of normal ovarian （NO） by immunohistochemical streptavidin/peroxidase method. Then the relationship between the expression of WT1 in human ovarian epithelial cancer and its clinical pathologic parameters was aggregate analyzed by SPSS13.0 software. Results： The expression of WT1 in epithelial ovarian cancer was higher than that in benign and normal ovarian tissue （ P 〈 0.01 ） , 28 （93%）of 30 serous carcinomas included in the study showed WT1 expression . Positive WT1 expression was correlated with poorer overall survival compared with negative WT1 expression. Patients with WT1 - positive tumors tended to have a higher grade and stage of tumor. The expression of WT1 was correlated with histological differentiation, FIGO stage, histology type and prognosis （P 〈 0.05 ） , but not correlated to age and the metastasis of lymph node（ P 〉 0.05 ）. Conclusion： The overexpression of WT1 may serve as a useful marker for assessment of the biological behavior of epithelial ovarian cancer and may helpful for assessing the malignancy prognosis .

关 键 词：肾母细胞瘤基因 卵巢肿瘤 免疫组织化学 

分 类 号：R737.31[医药卫生—肿瘤]