兔肝硬化肝癌立体定向照射后PCNA、GST-π表达及其意义  

Expression of PCNA and GST-π after stereotactic irradiation for liver cirrhosis and hepatocarcinoma in rabbits

作　　者：丁轶[1] 陈龙华[1] 吴德华[1] 孙爱民[1] 

机构地区：[1]南方医科大学南方医院放疗科,广东广州510515

出　　处：《南方医科大学学报》2007年第3期279-282,共4页Journal of Southern Medical University

基　　金：广东省自然科学基金(05004743)~~

摘　　要：目的应用已制备的兔肝硬化肝癌模型观察肝病状态下肝癌照射后放射性肝损伤的细胞因子的变化。方法肝硬化肝癌兔16只(实验组),随机分为两组,每组8只,分别给予单次剂量20、30Gy的立体定向照射;8只单纯接种肝癌兔(对照组),随机分为两组,每组4只,按上述方法分别给予立体定向照射,单次剂量20、30Gy。照射后3周分别处死实验组与对照组,应用EV二步法分别观察照射后实验组与对照组肝脏增殖细胞核抗原(PCNA)、谷胱甘肽-S-转移酶-π(GST-π)的变化。结果照射后PCNA、GST-π在癌周组织的表达:照射20Gy实验组比对照组的癌周组织表达高,两组间有显著性差异(P=0.010);照射30Gy实验组与对照组的癌周组织表达相似,两组间无统计学差异(P=1.000);实验组不同剂量照射后,高剂量组(30Gy)PCNA、GST-π的表达比低剂量组(20Gy)的表达低,两组间有显著性差异(P=0.010)。照射后PCNA、GST-π在肝癌组织的表达:实验组不同剂量照射,高剂量组(30Gy)的表达比低剂量组(20Gy)的表达低,两组间有显著性差异(P=0.010)。结论实验性肝硬化肝癌的照射效果单次剂量30Gy优于20Gy;PCNA、GST-π可作为评价肝硬化肝癌照射效果的参考指标。Objective To observe the changes of the cytokines following stereotactic irradiation for bepatocarcinoma with cirrhosis in rabbits. Methods Sixteen rabbits with liver cirrhosis and hepatocarcinoma （experimental group） were randomized into two equal groups to receive stereotactic irradiation at single dose of 20 and 30 Gy, respectively. Eight rabbits with hepatocarcinoma （control group） were divided into two equal groups and Ueated in identical manner. All the rabbits were kiUed 3 weeks after irradiation, and EV two-step method was used to observe the cytokine changes of proliferating cell nuclear antigen （PCNA） and glutathione S-transferase pi （GST-π） after irradiation. Results After irradiation, PCNA and GST-π expression showed significant difference in the adjacent liver tissue between the experimental and control rabbits with irradiation at 20 Gy （P=0.010）, but not with the irradiation dose of 30 Gy （P=1.000）. Irradiation at different doses resulted in significant difference in the cytokine expression in the experimental rabbits （P=0.010）. In the liver tissue exposed to irradiation, different irradiation doses resulted in significant difference in PCNA and GST-π protein expression （P=0.010）. Conclusions For hepatocacinoma with cirrhosis in rabbits, radiation at the single dose of 30 Gy produces better response than 20 Gy, and PCNA and GST-π may serve as good indexes for evaluating the therapeutic effect.

关 键 词：肝硬化 肝瘤 立体定向照射 增殖细胞核抗原 谷胱甘肽-S-转移酶-Π 

分 类 号：R735.7[医药卫生—肿瘤]