中性蛋白酶基因诱导型表达分泌载体的构建  

Construction of an Inducible Expression and Secretion System for Neutral Protease in Bacillus subtilis

作　　者：张敏[1] 赵丛[1] 杜连祥[1] 路福平[1] 蔡兴旺[1] 

机构地区：[1]天津科技大学生物工程学院天津市工业微生物重点实验室,天津300457

出　　处：《中国生物工程杂志》2007年第3期105-109,共5页China Biotechnology

摘　　要：利用PCR方法分别扩增出sacB基因的启动子?信号肽序列(sacR)和枯草芽孢杆菌中性蛋白酶的前肽?成熟肽序列,将两者连接后克隆入载体pHP13中,构建了含有中性蛋白酶基因的诱导型表达分泌载体pHP13SN,再将其转化入枯草杆菌DB104,获得基因工程菌DB104(pHP13SN)。中性蛋白酶基因在蔗糖的诱导和sacR的调控下实现了分泌表达,并获得了具有生物学活性的中性蛋白酶。The promoter and signal peptide sequence of sacB gene （sacR gene） has been amplified by PCR. An inducible expression and secretion vector pHP13SN has been constructed with this amplified sequence, which was ligated with the pro-peptide and mature peptide of neutral protease gene on the vector pHP13. Transforming Bacillus subtilis DB104 with the vector pHP13SN, and the recombinant strain DB104（pHP13SN） can be got. The neutral protease gene has been expressed by the inducement of sucrose and the regulation of sacR,and the production has been secreted with bioactivity.

关 键 词：枯草芽孢杆菌 中性蛋白酶 诱导表达 

分 类 号：Q785[生物学—分子生物学]