基于优质早籼稻品种佳辐占的遗传图谱的构建  

Rice Genetic Map Construction Based on Elite Indica rice,Jiafuzhan

作　　者：江良荣[1] 黄建勋[1] 张凯[1] 黄育民[1] 王侯聪[1] 宋思扬[1] 周克夫[1] 

机构地区：[1]厦门大学生命科学学院水稻遗传育种课题组,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2007年第2期262-267,共6页Journal of Xiamen University：Natural Science

基　　金：国家"863"项目(2002AA211091);厦门大学科技创新项目(XDKJCX20051001)资助

摘　　要：以优质常规稻佳辐占为父本,分别以广陆矮4号和明恢86为母本,构建两个重组自交系(以下简称为“广佳”群体和“明佳86”群体).利用559对简单重复序列(SSR)引物对亲本进行多态性分析,获得佳辐占和广陆矮4号、佳辐占和明恢86亲本间有差异的引物分别201对和186对,多态率分别达35.95%和33.33%.利用这些引物构建了两张水稻遗传图谱,其中广佳图谱包含127对SSR标记,全长约1 015.7 cM,平均标记间距为8 cM;明佳86图谱包含131对SSR标记,全长约1 263.6 cM,标记平均间距为9.6 cM.遗传图谱的构建便于研究佳辐占优质性状的遗传规律、外观品质性状间的内在关系,以期为分子标记辅助选育细长、大粒、优质的水稻新品种打下基础.同时,这是两个基于重组自交系的图谱,可长期用于群体内各种性状的遗传规律分析及QTL定位.Two recombination inbred lines （RIL） derived from Jianfuzhan× Guanglu＇ai 4 hao and Jiafuzhan × Minghui 86 （abbr, GJ and MJ86）were constructed. 559 pairs of SSR markers were used to analyze the polymorphism between Jianfuzhan and Guanglu＇ai 4 hao or Jiafuzhan and Minghui 86. It was shown experimently that there were 201 and 186 pairs of polymorphism markers respectively and their polymorphism ratio are 35.950% and 33.33% respectively. Two rice genetic maps were constructed based on these markers. The GJ map covered the rice genome about 1 015.7 cM with 127 SSR,whose average interval between two markers on the map was about 8 cM. The MJ86 map covered the rice genome about 1 263.6 cM with 131 SSR. The average interval was about 9.6 cM. These maps could be used for researching the genetic rules of Jiafuzhan elite traits and the inner relations among rice appearance traits,and also they could pave the road to breed new variety of high yield and elite traits by molecular marker-assisted breeding. As both the maps are from RIL, they could be used for the genetic rule analysis and QTL mapping of other traits,at any time.

关 键 词：水稻 重组自交系 遗传图谱 简单重复序列 

分 类 号：Q341[生物学—遗传学]