癌症差异蛋白质组学研究中样品分离和鉴定分析技术  

Separation and identification progress in cancer comparative proteomics

作　　者：李选文[1] 陈平[1] 张丽军[1] 梁宋平[1] 

机构地区：[1]湖南师范大学生命科学学院蛋白质化学与蛋白质组学研究室,长沙410081

出　　处：《生命科学》2007年第1期97-103,共7页Chinese Bulletin of Life Sciences

基　　金：国家"973"课题资助项目(2001CB5120)

摘　　要：随着人类基因组测序的完成,癌症研究的重点从基因组学转移到蛋白质组学研究中。癌症研究中的差异蛋白质组学技术也飞速发展,包括癌症样品制备、分离,蛋白质鉴定分析、蛋白质组定量研究和翻译后修饰研究等。这些技术极大地推动了与癌症相关的差异蛋白质组学研究,使蛋白质组学在癌症早期诊断、治疗,监测以及发现新药物治疗靶标方面发挥更大的作用。本文主要综述了近年来癌症差异蛋白质组学研究中样品分离和鉴定分析技术。With the human genome sequence now determined, the field of cancer research is moving beyond genomics to proteomics. The comparative strategies involving in the cancer proteomic research have been developed, including cancer sample preparation, proteins or peptides separation, protein identification, bioinformatics, protein quantification and so on. All these will accelerate the identification of cancer markers that could be important in early detection, diagnosis and monitoring efficacy of treatment, and also the development of protein-based, cancer-specific targets, which have a great effect on cancer medicine. In this review, we focused on the sample separation and identification progress in cancer comparative proteomics.

关 键 词：癌症 差异蛋白质组 二维电泳 色谱 定量蛋白质组 

分 类 号：Q51[生物学—生物化学] R73[医药卫生—肿瘤]