大鼠肠缺血-再灌注损伤中FGFR2表达的规律  

Expression of acidic fibroblast growth factor in rat gut ischemia/reperfusion injury

作　　者：翁立新[1] 付小兵[2] 

机构地区：[1]内蒙古医学院病理学教研室,呼和浩特010059 [2]解放军总医院,北京100037

出　　处：《临床与实验病理学杂志》2007年第1期69-72,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家重大基础研究规划资助项目(G1999054204);国家自然科学基金重点项目(30230370);国家自然科学基金面上项目(30170966);国家863资助项目(2001AA215131)

摘　　要：目的观察酸性成纤维细胞生长因子受体2(acid fibroblast growth factor receptor,FGFR2)在肠缺血-再灌注损伤中表达规律。方法以大鼠肠系膜上动脉(SMA)夹闭造成肠缺血-再灌注损伤模型,并将动物随机分为假手术组(C组)、生理盐水对照组(R组)、改构体aFGF治疗组(F组)。除假手术组外,其余各组动物均于缺血45min后于2、6、12、24h活杀,取小肠组织标本,免疫组化和RT-PCR检测FGFR的表达规律。结果在正常大鼠,FGFR分布在小肠绒毛上皮细胞的肠腔侧、侧壁和小肠隐窝朝向隐窝腔的一侧的细胞膜上。缺血和再灌注的初期,FGFR的表达未发生明显变化,随着再灌注时间的延长逐渐增强。FGF使FGFR的表达有明显的增强和提前表达。缺血和再灌注使FGFR mRNA的表达迅速增加,在再灌注后6h达到高峰。F组FGFR mRNA的表达较R组相应时间点显著增加(P<0.05)。结论FGFR在肠缺血-再灌注损伤的修复中起积极作用。Purpose To identify the regulation of change of acidic fibroblast growth factor receptor in rat gut ischemia-reperfusion （ I/ R） injury after the administration of acidic fibroblast growth factor （aFGF）. Methods Rat I-R injury was produced by clamping the superior mesenteric artery （SMA）, and the animals were divided randomly into sham operation group（ C）, normal saline control group （R）, reconstructive human aFGF treatment group（F）. Blood samples and tissue specimens were collected at 2, 6, 12 ,and 24 hours after reperfusion. The expression of FGFR and the levels of FGFR mRNA were detected. Results The expression of FGFR mRNA increased gradually after I/R injury and peaked at 6 hours after reperfusion. The expression level in group F was higher than that in group R. Conclusion The protective effect of aFGF on the intestine after I/R injury might be mediated by the activation of FGF receptor.

关 键 词：酸性成纤维细胞生长因子 受体 缺血-再灌注损伤 大鼠 

分 类 号：R364[医药卫生—病理学]