Leptin降低脓毒症后肺肠FABP水平及其机理  

LEPTIN DECREASES POST-SEPTIC PULMONARY AND INTESTINAL FABP LEVELS AND ITS MECHANISM

作　　者：颜光涛[1] 郝秀华[1] 林季[1] 薛辉[1] 张凯[1] 王录焕[1] 

机构地区：[1]解放军总医院基础医学研究所生化研究室,北京100853

出　　处：《中国应用生理学杂志》2007年第1期82-86,共5页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金面上项目(39970717)

摘　　要：目的:研究脓毒症对肺肠组织脂肪酸结合蛋白(FABP)水平及相关酶活性的影响,探讨FABP在急性炎症反应中的作用。方法:建立小鼠盲肠结扎致脓毒症模型,采用放射免疫分析法和96孔分光光度分析法分别检测肺肠组织匀浆液中FABP及髓过氧化物酶(MPO)、超氧化物歧化酶(SOD)等与自由基清除相关的酶的水平、同时以HE染色方法观察肺肠组织病理组织学改变。结果:脓毒症6 h和12 h后肺组织匀浆放免测定脂肪酸结合蛋白(FABP)水平显著上升(108.11±94.03vs204.98±70.72,67.22±19.47vs154.29±60.14)。腹腔给药Leptin(0.1 mg/kg)、消炎痛(2 mg/kg)后12 h可见肺组织FABP水平又下降并低于脓毒症水平(P<0.05)。同样,脓毒症后12 h肠组织FABP升高,Leptin保护后水平显著下降(419.80±80.06vs191.09±96.75),而消炎痛保护未见显著FABP下降。同时,脓毒症后肺肠组织内MPO、SOD活性改变随时间有不同变化,Leptin和消炎痛保护对其的影响与FABP改变并无显著的相关性。结论:Leptin可以保护脓毒症肺肠等重要脏器的功能,表现为细胞损伤标志FABP的水平可以明显低于未保护组水平,但可能同组织MPO、SOD等氧自由基清除因素并不相关。Aim: To detect the efect of sepsis on fatty acid binding proteins(FABP) levels and corresponding enzymes in lung and intestine of mice,and to explore the role for FABP in acute inflammation.Methods: A sepsis model of mice made with cecum deligation and perforation was established,and a radioimmunoassay for FABP and 96-well spectrophotometry assays for myeloperoxidase(MPO) and superoxide dismutase(SOD)which were related with clearance of free radicals,were used to detect their levels in lung and intestine homogenized fluids.Hematoxylin-eosin stain was used simultaneously to check the histopathologic chanes of both tissues.Results: Compared with sham group(108.11±94.03 and 67.22±19.47 ng/ml) 6 h and 12 h after sepsis,FABP levels in lung and intestine were significantly higher(204.98±70.72 and 154.29±60.14 ng/ml),respectively.Twelve hours afeter leptin(0.1 mg/kg i p) and indomethacin(2 mg/kg i p) injection,lung FABP level decreased and was lower than septic group(P<0.05).Moreover,12 h after sepsis intestinal FABP increased,but it decreased after leptin injection(419.80±80.06 vs 191.09±96.75 ng/ml),while indomethacin injection had no such effect.MPO and SOD activities in lung and intestine changed accordingly with time after sepsis,the effect of leptin and indomethacin injections on it had no significant correlation with FABP changes.Conclusion: Leptin can protect vital organ functions such as lung and intestine after sepsis,as FABP levels,the cellular injury marker,were significantly lower than groups wi-thout injection.And this effect might have no correlation with the clearance factors of oxigenic free radicals such as MPO and SOD.

关 键 词：LEPTIN 脓毒症 脏器功能 脂肪酸结合蛋白 髓过氧化物酶 超氧化物歧化酶 

分 类 号：R392.32[医药卫生—免疫学]