川芎嗪对家兔肺缺血/再灌注损伤时Fas/FasL基因表达的影响  

EFFECT OF LIGUSTRAZINI ON EXPRESSION OF FAS/FASL IN PULMONARY INJURY INDUCED BY ISCHEMIA/REPERFUSION IN RABBITS

作　　者：王万铁[1] 王晓扬 陈瑞杰[3] 郝卯林[1] 王方岩[1] 陈锡文[4] 方周溪[5] 

机构地区：[1]温州医学院病理生理学教研室,浙江温州325027 [2]金华职业技术学院医学院病理学教研室,浙江金华321000 [3]温州医学院附属第二医院药剂科,浙江温州325027 [4]温州医学院附属第二医院实验动物中心,浙江温州325027 [5]温州医学院附属第二医院电镜室,浙江温州325027

出　　处：《中国应用生理学杂志》2007年第1期87-91,共5页Chinese Journal of Applied Physiology

基　　金：浙江省跨世纪学术和技术带头人培养基金(992086);温州市"551人才工程"培养基金(98113);浙江省教育厅科研基金(20000670);温州市科技计划重点项目(Y2005A080)

摘　　要：目的:探讨川芎嗪对肺缺血/再灌注损伤(PI/RI)时Fas/FasL基因表达的影响。方法:采用在体兔单肺原位缺血-再灌注模型。实验兔90只,随机均分为假手术对照组(Sham)、肺缺血/再灌注组(I/R)和肺缺血/再灌注加川芎嗪治疗组(LGT)。每组又分为再灌注1h3、h、5 h三个亚组,每个亚组10只,分别于再灌注1 h3、h、5 h三个时点取左肺组织,观察Fas/Fas配体(Fas/FasL)mRNA定位表达、凋亡指数(AI)、肺组织湿、干重比(W/D)、肺损伤组织学定量评价指标(IQA)及光镜、电镜下的组织形态学改变。结果:肺再灌注1 h、3 h、5 h,LGT组Fas/FasLmRNA在肺小动脉内(外)膜、肺小静脉内膜、肺泡上皮及肺支气管上皮弱阳性表达,与I/R组同一时点比较阳性表达明显减弱;AI、W/D和IQA值显著低于I/R组(P<0.01和P<0.05);肺组织形态学异常改变不同程度减轻。结论:川芎嗪可下调肺组织Fas/FasL mRNA的表达而减轻细胞凋亡,对PI/RI发挥积极的防护作用。Aim： To investigate the effect of ligustrazini （LGT） on expression of Fas/FasL mRNA during pulmonary ischemia/reperfusion injury （PI/RI） in the rabbits. Methods： Single lung ischemia/reperfusion animal modal was used in this study. The rabbits were randomly divided into three groups （ n = 30, in each） ： sham operated group （Sham）, I/R group （I/R） and I/R ＋ LGT group（ I/R ＋ LGT）. Changes of several parameters which included apoptotic index （AI）, wet to dry ratio of lung tissue weight（W/D） and index of quantitative assessment of histologic lung injury（IQA） were measured at 1h, 3h, 5h after reperfusion in lung tissue. Meanwhile the location and expression of Fas/FasL mRNA were observed. Lung tissue was prepared for light microscopic and dectron microscopic observation at 1 h, 3 h, 5 h after reperfusion. Results： As compared with group I/R, Fas/FasL mRNA slightly expressed in intirna and extima of small pulmonary artery, alveoli, and bronchiole epithelia in group LGT. The values of AI ,W/D and IQA showed significantly lower in group I/R ＋ LGT than that in group I/R at 1 h, 3 h, 5 h after reperfusion in lung tissue （P 〈 0.01 and P 〈 0.05）. Meanwhile,abnormal changes of the lung tissue in morphologically were lessen markedly in group I/R ＋ LGT. Conclusion： Ligustrazini has notable protective effects on PI/RI in rabbits by inhibiting Fas/FasL mRNA express in lung tissue and decreasing apoptosis.

关 键 词：缺血/再灌注损伤 肺 FAS/FAS配体 细胞凋亡 川芎嗪 

分 类 号：R363.2[医药卫生—病理学]