Preparation and Characterization of β-Cyclodextrin Derivatized Ovalbumin Used as Chiral Selector in Pressure Capillary Electrochromatography  

作　　者：禹玉洪 唐力 戴荣继 邓玉林 傅若农 

机构地区：[1]School of Life Science and Technology,Beijing Institute of Technology, Beijing 100081, China [2]School of Life Science and Technology,Beijing Institute of Technology, Beijing 100081,China,Department of Environmental Science, Zhejiang Wanli University,Ningbo 315100,China [3]School of Science, Beijing Institute of Technology, Beijing 100081, China

出　　处：《Journal of Beijing Institute of Technology》2007年第1期108-111,共4页北京理工大学学报（英文版）

基　　金：the National Natural Science Foundation of China(20275004)

摘　　要：Synthesis and properties of β-cyclodextrin derivatized ovalbumin used as chiral selector were investigated, β-cyclodextrin derivatized ovalbumin was synthesized using β-cyclodextrin and ovalbumin in the presence of ethylene glycol diglycidyl ether in boric acid buffer at pH value 8.7 at 37℃. Amino group was coated on the internal surface of the silica capillary by sol-gel technology with triethoxylmethylsiloxane and （3-aminopropyl）trimethoxysiloxane. Covalent binding of g-cyclodextrin derivatized ovalbumin was performed by glutaraldehyde. Enantiomers of chlorpheniramine, phenylalanine and atropine were separated by pressure capillary electrochromatography column coated with β-cyclodextrin derivatized ovalbumin.Synthesis and properties of β-cyclodextrin derivatized ovalbumin used as chiral selector were investigated, β-cyclodextrin derivatized ovalbumin was synthesized using β-cyclodextrin and ovalbumin in the presence of ethylene glycol diglycidyl ether in boric acid buffer at pH value 8.7 at 37℃. Amino group was coated on the internal surface of the silica capillary by sol-gel technology with triethoxylmethylsiloxane and （3-aminopropyl）trimethoxysiloxane. Covalent binding of g-cyclodextrin derivatized ovalbumin was performed by glutaraldehyde. Enantiomers of chlorpheniramine, phenylalanine and atropine were separated by pressure capillary electrochromatography column coated with β-cyclodextrin derivatized ovalbumin.

关 键 词：capillary electrochromatography OVALBUMIN chiral separation Β-CYCLODEXTRIN 

分 类 号：O636.1[理学—高分子化学]