MTBE降解菌的分离及降解动力学分析  

Screening for MTBE Degrading Bacteria and Its Degrading Kinetics

作　　者：张瑞玲[1] 李鑫钢[1] 欧志龙[1] 姜斌[1] 廉景燕[2] 

机构地区：[1]天津大学化工学院,天津300072 [2]天津理工大学生物与化学工程学院,天津300191

出　　处：《农业环境科学学报》2007年第1期301-305,共5页Journal of Agro-Environment Science

基　　金：国家自然科学基金(20276048);天津市自然科学基金(06YFJMJC06800)

摘　　要：从甲基叔丁基醚(MTBE)污染场地的土壤样品中分离得到一株高效降解菌株A-3,经形态及生理生化鉴定和16SrDNA基因序列分析,该菌株鉴定为金黄杆菌(Chryseobacteriumsp.)。对该菌株降解MTBE的特性进行了研究,结果表明:添加100mg·L-1酵母膏及0.05～0.1mg·L-1Co2+对细菌的生长及MTBE的降解具有一定的促进作用。对该菌的降解动力学进行了分析,MTBE的降解符合抑制动力学Haldane模型,最大降解速率Vmax=0.11d-1,半饱和常数ks=161.7mg·L-1,抑制常数k1=68.2mg·L-1。该菌株对于烃类等物质具有广泛的利用能力,其在环境中的应用具有一定的意义。Methyl tert-butyl ether （MTBE）, as an important gasoline additive, possessing serious environmental health problems, has already caused the attention of the world. Its unique physical and chemical properties, for example, its low adsorption on matter and its high solubility in water give MTBE a great persistence and mobility in the groundwater. Biological treatment of soil and groundwater is expected to be the preferred cost-effective remediation technique. A new strain that can degrade MTBE was isolated from MTBE plant soil by plating on solid medium containing MTBE as the sole carbon and energy source. Base on the morphological and physiological biochemical characteristics and its 16S rDNA gene sequence and data base comparison, the new strain A-3 belonged to the Chryseobacterium sp. The positive effect of yeast extract and Co^2＋ during growth of A-3 on MTBE were demonstrated. Adding 100 mg·L^-1 yeast extract and 0.05-0.1 mg·L^-1 Co^2＋ could promote the growth of the strain and degradation of MTBE. Degrading kinetics of MTBE fitted the model of Haldane, with the maximum substrate utilization rate 0.11 d^-1, the half-saturation concentration 161.7 mg·L^-1 and inhibitory constant 68.2 mg·L^-1. The strain A-3 can utilize diverse alkane and nonakane substrates efficiently, which is useful to its application on bioremediation process. The Chryseobacterium sp., as a new genus of bacteria degrading MTBE, would give a new insight about biodegradation process of MTBE and the strain would be promising in treating mixture wastewater.

关 键 词：甲基叔丁基醚 生物降解 酵母膏 钴离子 降解动力学 

分 类 号：X172[环境科学与工程—环境科学]