紫外光诱导哈茨木霉产生对多菌灵抗药性的菌株  

Carbendazim Resistance Strains from Trichoderma Harzianum Induced by UV-light

作　　者：田连生[1] 

机构地区：[1]河北省生物研究所,河北石家庄050081

出　　处：《农业环境科学学报》2007年第1期318-321,共4页Journal of Agro-Environment Science

基　　金：河北省科技攻关项目(02245504D)

摘　　要：在含多菌灵药物培养基上,采用紫外光重复诱导处理的方法,对拮抗性哈茨木霉T24进行改良,选育出对多菌灵具有显著耐药性的菌株T24-4和T24-6。结果表明,多菌灵浓度为300mg·L-1时,对突变菌株T24-4和T24-6的抑制率分别为9.4%和3.0%,同时两菌株对速克灵和甲基托布津也有交互抗性。经过菌和药协同作用研究发现:木霉菌T24-6菌剂与多菌灵重量配比为8∶2时,协同抑菌率高达85.0%,明显高于分别单独使用T24-6和多菌灵的抑菌率(70.3%和69.6%)。分别用菌和药,药,菌1000倍稀释液进行草莓活体拮抗性测定,其对草莓灰霉病的防治效果分别为83.9%、67.9%和64.2%,进一步表明菌和药协同作用效果优势更明显。在使用木霉T24-6菌剂防治农作物病害时,添加少量的多菌灵,可降低植物病原菌的活性和侵染力,从而提高生防效果。To realize beneficial interaction of biopestieide and chemical pesticide, the resistant Trichoderma harzianum strain T24-6 against Carbendazim was obtained by UV-ligbt repeatedly inducing and Carbendazim stress on PDA medium. The inhibition rate of Carbendazim to T24-6 was 3.0% when Carbendazim concentration was 300 mg·L^-1. Five treatments, Trichoderma, Carbendazim, three combinations of Trichoderma and Carbendazim with different weight rates 9：1, 8：2 and 7：3 respectively, were designed in the experiment. In every treatment, the inhibition rate and the antagonism of vivo-strawberry were tested. The inhibition efficiency of Botrytis einerea was 85% under the treatment with application of Trichoderma and Carbendazim at 8：2, higher than that in the treatment of applying Triehoderma T24-6 （70.3%） and Carbendazim（69.6%） separately. Similarly, the efficiency of controlling strawberry mold disease caused by Botrytis einerea was 83.9% by application of Trichoderma and Carbendazim at 8：2. The controlling effect was also better than the treatment of Trichoderma（67.9%） and Carbendazim （64.2%） separately. These results further demonstrated that the beneficial interaction of Trichoderma and Carbendazim was more advantageous. So, when using Trichoderma strain T24-6 to prevent and control crop diseases, adding a small amount of Carbendazim not only reduce the activity and ability of infection of plant pathogens but also improve the effects of biological control.

关 键 词：紫外光诱导 哈茨木霉 耐药性 拮抗性 

分 类 号：X172[环境科学与工程—环境科学]