尼美舒利对结肠癌细胞株PGE2及VEGF表达的影响  

Effect of Nimesulide on the Expression of PGE2 and VEGF of Human Colon Cancer Cells

作　　者：尹逊海[1] 姜爱民[1] 张秀峰[1] 梁桃[1] 

机构地区：[1]哈尔滨医科大学附属第一临床医学院消化内科,黑龙江哈尔滨150001

出　　处：《中国临床医学》2007年第1期29-30,共2页Chinese Journal of Clinical Medicine

摘　　要：目的:探讨选择性环氧化酶2(COX-2)抑制剂对COX-2高表达的结肠癌细胞株HT-29增殖和凋亡的影响,明确以COX-2为靶点治疗结肠癌的作用途径。方法:将选择性COX-2抑制剂尼美舒利(nimesulide)按不同浓度作用于结肠癌细胞系HT-29,用MTT(四甲基偶氮唑蓝比色法)法分别于0、24、48、72h检测细胞增殖状态;流式细胞仪观察尼美舒利对细胞凋亡的影响,进一步采用ELISA法检测药物作用后前列腺素E2(PGE2)的表达,免疫组化法测定内皮细胞生长因子(VEGF)表达阳性率。结果:尼美舒利对结肠癌细胞系HT-29呈时间、剂量依赖性方式抑制细胞增殖,促进其凋亡。PEG2及VEGF表达水平随作用时间延长而下降。结论:选择性COX-2抑制剂可能通过PGE2与VEGF途径影响结肠癌细胞HT-29的增殖与凋亡,是其治疗结肠癌的分子机制。Objective：To investigate the influence of nimesulide, a selective cyclooxygenase-2（COX-2） inhibitor, on the prolfieration and apoptosis of colorectal cancer cell HT-29, and to explore it＇s potential mechanism to treat colorectal carcinoma, Methods：Cultured HT-29 cells were treated with nimesulide. MTT assay and flow cytometry were used to measure the prolifer- ation and apoptosis in 0,24,48,72 h respectively. The expression of PGE2 and VEGF was detected by ELISA and immunohistochemistry methods separately after treatment With nimesulide. Results： Nimesulide inhibited the cells proliferation and induced apoptosis in a dose- and time-dependent manner, and resulted in a significant down-regulation of PGE2 and VEGF. Conclusion：Our results showed that Nimesulide may inhibit the proliferation and induce apoptosis of colon cancer cell through decreasing expression of PGE2 and VEGF. This may be a new interfering target of selective COX-2 inhibitor on treating colon cancer.

关 键 词：结肠癌 环氧舍酶抑制荆 前列腺素E2 内皮细胞生长因子 增殖 凋亡 

分 类 号：R735.35[医药卫生—肿瘤]