赤芍水提物对肝星状细胞株HSC-T6促凋亡作用的实验研究  

The Induce Effects of Chishao Distilled Substances on the Apoptosis of HSC-T6 Cells

作　　者：韩海啸[1] 李军祥[1] 刘大新[2] 朱陵群[3] 崔巍[3] 

机构地区：[1]北京中医药大学东方医院消化内科,北京100078 [2]北大医院中医针灸科,北京100000 [3]北京中医药大学东直门医院中医内科学重点实验室,北京100700

出　　处：《深圳中西医结合杂志》2007年第1期1-5,共5页Shenzhen Journal of Integrated Traditional Chinese and Western Medicine

基　　金：教育部高等学校博士点基金资助项目(20040026016)

摘　　要：目的应用流式细胞法,研究灌服赤芍水提物后的犬血清对乙醛造模后的肝星状细胞株HSC-T6细胞的促凋亡作用。方法采用乙醛造模后的肝星状细胞株HSC-T6作为体外研究模型,以赤芍水提物给彼格犬一次性灌胃,取给药前的血清、给药后1h、2h、3h、5h血清作为实验药物血清。以流式细胞法(flow cytometry)分别检测以上采血时间点上2%浓度的药物血清对乙醛造模后的肝星状细胞株HSC-T6作用72h后的促凋亡作用。结果2h、3h两个时间点的含药血清能明显促进HSC-T6细胞的凋亡,其中2h2%含药血清的促凋亡率5.71%,3h2%含药血清的促凋亡率5.73%。和同样条件下用空白血清培养的HSC-T6细胞相比其凋亡比例显著性增加(均为P<0.05)。在药物血清的作用下两个时点组的HSC-T6细胞在G0/G1期细胞比例明显上升(P<0.05);而S期细胞显著减少(P<0.05);但G2/M期并没有一致性的变化。结论两个时点的药物血清对HSC-T6细胞都有显著的促凋亡作用,且能显著增加HSC-T6细胞在G0/G1期的比例及显著降低S期的比例,这可能是其抑制HSC-T6细胞增殖及促凋亡的原因之一。Objective By means of flow cytometry, we studied the apoptosis-promoting effects of the dog serum fed by Chishao distilled substances on hepatic stellate cell （HSC-T6） builded model by acetaldehyde. Methods The experiments adopted eternalized human hepatic stellate cell （HSC-T6） builded model by acetaldehyde,for study in vitro. Feeding the dog with Chishao distilled substances one time, we took the serum before feeding and at 1-hour, 2-hour, 3-hour and 5-hour different points of time, when the medicated serums had been proved best inhibiting effects, after feeding as the medicated serum for experiment. By means of flow cytometry, we examined the apoptosis-promofing effects in 72 hours on HSC-T6 cells by 2% concentration of medicated serum at different points of time. Results The medicated serums has significant effects of promoting HSC-T6 apoptosis at these two points of time. Compared with non-medicated serums under the same conditions, the proportion of apoptosis had a significant increase （P 〈 0.05）. At the 2-hour, 3-hour point, the apoptosis rate of it is 5.71%, 5.73%. HSC-T6 cells, proportion was increased in G0/G1 period （P 〈 0.05） and decreased in the S period （P 〈 0.05）, while no consistant changes are observed in G2/M period. Conclusion At these two points of strongest inhibiting effects, the serums had significant promoting-apoptosis effects on HSC-T6. Dramatically decreasing HSC-T6 cells＇ proportion in S period and increasing it in the G0/G1 period were probably responsible for the effects of inhibiting the proliferation of HSC-T6 cells and promoting their apoptosis.

关 键 词：赤芍水提物 肝星状细胞株HSC—T6 凋亡 酒精性肝纤维化 细胞培养 流式细胞术 

分 类 号：R575.2[医药卫生—消化系统]