急性髓细胞性白血病病人TCRζ链基因表达特点  

The Feature of TCR-Zeta Chain Expression in Patients with Acute Myeloid Leukemia

作　　者：陈思[1] 李扬秋[1] 陈少华[1] 杨力建[1] 吴秀丽[1] 岑东芝[1] 

机构地区：[1]暨南大学医学院血液病研究所,广州510632

出　　处：《循证医学》2007年第1期54-57,共4页The Journal of Evidence-Based Medicine

基　　金：国家自然科学基金项目(30270579;30424003);广东省自然科学基金重点项目(23001);血液病学国务院侨办重点学科建设基金

摘　　要：目的建立实时定量PCR方法检测TCRζ链表达水平的方法,了解急性髓细胞性白血病病人外周血TCRζ链基因表达水平。方法采用SYBR Green Ⅰ荧光定量PCR和相对定量分析法检测31例急性髓细胞性白血病患者(M1型6例,M2型15例,M3型10例)和30例正常人外周血的单个核细胞的TCRζ链表达情况,以β2微球蛋白基因(β2M)作为内参,根据相对定量公式2-△△Ct计算急性髓细胞性白血病患者与正常人TCRζ链表达差异倍数。结果与正常人相比,急性髓细胞性白血病患者TCRζ链表达特点可以分为表达缺失(16%)、表达下降(26%)和表达上升(58%)三种情况。而三种不同亚型急性髓细胞性白血病之间的TCRζ链表达模式相似。结论本研究成功建立了SYBR Green Ⅰ荧光实时定量PCR和相关定量分析TCRζ链表达水平技术,并首先报道了急性髓细胞性白血病病人TCRζ链表达水平的变化。Objective To establish a real-time PCR technique for detection and quantify of TCRξ chain expression and to investigate TCRξ chain gene expression level in patients with acute rnyeloid leukemia （AML）. Methods Real-time PCR with SYBR Green Ⅰ technique was used for detecting TCRξ chain expression level in peripheral blood mononuclear cells of 31 patients with AML [including 6 cases with AML-M1 subtype （M1）, 15 cases with M2, 10 cases with M3] and 30 novmal individuals. β2-microglobulin gene （β32M） was used as an endogenous reference. Relative changes in TCRξchain expression level were used by the 2^-△△α method between patients with AML and normal individuals. Results In comparison with normal individuals groups, the feature of TCRξ chain expression level could be divided into 3 different groups with absent expression （16%）, down expression （26%） and over expression （58%） respectively. Similar expression pattern of TCRξ chain expression level was found in the 3 subtypes of AML. Conclusions The SYBR Green Ⅰ read-time technique for quantitative detection of TCRξ chain expression levels was established successfully. This is, to our knowledge, the first description in the expression feature of TCRξ chain gene in AML.

关 键 词：TCRξ链 实时定量PCR 急性髓细胞性白血病 

分 类 号：R733.71[医药卫生—肿瘤]