纳豆激酶脂质体的制备及其体外释放  

Preparation and in Vitro release of Nattokinase Liposomes

作　　者：张霞[1] 尹宗宁[1] 王怡鑫[1] 

机构地区：[1]四川大学华西药学院,成都610041

出　　处：《中国药学杂志》2007年第4期279-282,共4页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(30371696)

摘　　要：目的研究纳豆激酶脂质体的制备工艺以及体外释放。方法以薄膜分散法制备纳豆激酶脂质体,以包封率为指标正交试验优化其制备工艺,观测其形态、粒径和Zeta电位,并进行体外释放实验。结果卵磷脂(100 mg)、胆固醇(20 mg)、纳豆激酶质量浓度(0.25 g.L-1)、PBS体积(5 mL)和减压蒸发时间(90 m in)时包封率最高,电子显微镜下可见多层囊状脂质体,测得3批纳豆激酶脂质体的平均包封率为53.44%,平均粒径为235.7 nm,Zeta电位为-46.82 mV,体外释放符合一级动力学释放规律,并在12 h内释放完全。结论纳豆激酶脂质体制备工艺简单、包封率高、成型良好,体外释放迅速。OBJECTIVE To optimize the formulation of nattokinase liposome and to study its in vitro release. METHODS The nattokinase liposomes were prepared by film dispersion method, its formulation was optimized by orthogonal design, and its entrapment ratio, form, size, Zeta potential and in vitro release were determined. RESULTS Entrapment ratio reached its highest value with lecithin（ 100 mg） ,cholesterol（20 mg） ,nattokinase（0. 25 g · L^-1） ,PBS（5 mL）and time（90 min）. Three batches of liposomes were prepared, multilamellar vesicles were seen by electron microscope, average entrapment ratio was 53.44%, average particle size was 235.7 nm, and Zeta potential was -46. 82 mV. In vitro release was fitted with first order kinetic equation, and nattokinase was completely released within 12 h. CONCLUSION The formulation and technique of nattokinase liposomes is easy, with high entrapment ratio, good form and quick in vitro release.

关 键 词：纳豆激酶 脂质体 溶栓 体外释放 

分 类 号：R943[医药卫生—药剂学]