亚甲基四氢叶酸还原酶A1298C多态性与胃癌易感性的关系  

Relationship between the Methylenetetrahydrofolate Reductase A1298C Genetic Polymorphisms and Susceptibility to Gastric Cancer

作　　者：符刚[1] 杨玉凤[1] 沈孝兵[1] 浦跃朴[1] 张娇[1] 

机构地区：[1]东南大学公共卫生学院劳动卫生与环境卫生学系,江苏南京210009

出　　处：《环境与职业医学》2007年第1期32-35,共4页Journal of Environmental and Occupational Medicine

基　　金：东南大学科技基金项目(编号:XJ019004)

摘　　要：[目的]探讨亚甲基四氢叶酸还原酶（MTHFR）基因多态性与胃癌易感性的关系及与环境因素的交互作用。[方法]收集南京市原发性胃癌患者169例，采用1：1病例．对照研究方法随机选取非消化系统疾病、非肿瘤病人为对照，进行流行病学调查，取外周血，采用限制性片段长度多态性方法（PCR—RFLP）分析研究对象MTHFR1298位点的多态性，并分析MTHFR1298基因多态性与烟酒嗜好在胃癌发生中的交互作用。[结果]MTHFRl298C等位基因在病例组与对照组中的频率分别为21．6％和13．0％，差异具有显著性（x^2=8．693，P=0．003，OR=1．841，95％CI=1．223—2．772）。携带MTHFR1298A／C基因型者患胃癌危险性增高（x^2=10．994，P=0．001，OR=2．160，95％CI=1．365—3．419），携带A／C基因型同时又吸烟者患胃癌的危险性是AM型不吸烟者的3．289倍（x^2=10．002，P=0．002，95％CI=1．573～6．880），携带A／C基因型不饮酒者患胃癌的危险性是携带A／A基因型不饮酒者的2．726倍（x^2=12．459，P〈0．001，95％CI=1．562—4．758），同时携带A／C基因型、吸烟、饮酒者患胃癌的危险性是携带A／A基因型、不吸烟、不饮酒者的2．394倍（x^2=3．933，P=0．047，95％CI=1．010—5．672）。[结论]MTHFR1298A/C基因型可增加胃癌易感性，并与吸烟、饮酒间存在交互作用。[ Objective ] To evaluate the association between methylenetetrahydrofolate reductase （ MTHFR ） genetic polymorphisms and gastric cancer susceptibility. [ Methods ] PCR-RFLP technique were used to detect the genetic poiymorphisms of MTHFR1298 among 169 cases of gastric cancer and an 1：1 control group of cancer-free and matched by age （ ± 5 years ） and sex. Each subject was asked to answer a questionnaire about epidemiology of gastric cancer and was blood sampled for analyzing the association between the interaction of MTHFR1298 genetic polymorphisms and smoking and drinking status and gastric cancer susceptibility. [ Results ] There＇s significant difference of the frequency of MTHFR1298C allele between case group and control group（X^2=8.693, P=0.003, OR=1.841,95%CI=1.223-2.772 ）. There＇s a higher frequency of MTHFR1298,4/C genotype in case group than control group（x^2=10.994, P=0.001, OR=2.160, 95%CI=1.365-3.419 ）. An interaction between MTHFR1298 genotypes and smoking and drinking status was observed. The risk of gastric cancer increased in individuals who both carriying A/C genotype and smoking comparing with those carrying A/A genotype and non-smoking（ x^2=10.002, P=0.002, OR=3.289, 95%CI= 1.573-6.880 ）. Among non-drinking people, those carrying A/C genotype had a higher susceptibility to gastric cancer than those carrying A/A genotype （x^2= 12.459, P〈0.001, OR=2.726, 95%CI=1.562-4.758 ）. People who carrying A/C genotype, smoking and drinking had a higher susceptibility to gastric cancer than those carrying A/A genotype, non-smoking and non-drinking （x^2=3.933, P=0.047, OR=2.394, 95%CI=1.010-5.672 ）. [ Conclusion ] A/C genotype of MTHFR1298 might contribute to gastric cancer susceptibility and interact with smoking and drinking.

关 键 词：亚甲基四氢叶酸还原酶 基因多态性 胃癌 病例-对照研究 

分 类 号：R394[医药卫生—医学遗传学]