大鼠单克隆抗体夹心ELISA法检测肝病病人血清中P21^（ras）蛋白  

Establishment of Rat McAb Sandwich ELISA and Determination of p21^(ras)Protein in Patients with Hepatopathy

作　　者：程新宪 易志群 

机构地区：[1]解放军323医院

出　　处：《细胞与分子免疫学杂志》1996年第1期29-31,共3页Chinese Journal of Cellular and Molecular Immunology

摘　　要：本文应用抗癌基因ｒａｓ表达产物Ｐ２１ｒａｓ蛋白的大鼠单克隆抗体建立的双抗体夹心ＥＬＩＳＡ法，检测了肝癌及癌病变前病人血清中ｐ２１ｒａｓ蛋白的浓度。所获阳性检出率分别为：慢性乙型肝炎为１３．０％（１２／９２），肝硬化为２７．８％（１０／３６）和肝癌为５０．０％（８／１６）。另外，对试验方法的敏感性、特异性及稳定性，以及检测Ｐ２１ｒａｓ蛋白与ＡＦＰ的关系进行了评价。结果表明，Ｐ２１ｒａｓ蛋白的过高表达发生在肝细胞癌变之前。提示在慢性乙型肝炎及肝硬化病人血清中检出Ｐ２１ｒａｓ蛋白，是预测肝细胞癌变的危险信号。Using a sandwich ELISA based on rat McAb against p21ras protein, product encoded by rasoncogene, we have detected serum p21rasprotein level in patients with liver cancer and in precancerouspateints. PO8itive detection rates of p21ras protein obtained in the Patients were 13. 0% (12/92) forchronic hepatitis B, 27. 8 % (12/36) for cirrhosis and 50. 0 % (8/16) for liver cancer, respectively.Furthermore,specificity, sensitivity and stability of the ELISA and correlation between p21ras proteinand alpha fedal protein (AFP) were also assessed.The results indicated that increase of serum p21rasprotein level occured in precancerous patientS with chronic hepatitis B and cirrhosis might be a dangerous signal of predicting hepetocellular canceration.

关 键 词：肝肿瘤 RAS癌基因 P21^RAS蛋白 ELISA 检测 

分 类 号：R735.704[医药卫生—肿瘤] R446.62[医药卫生—临床医学]