质谱技术研究蓝斑背肛海兔胸膜神经节连索外表层多肽组成与分布  

Peptide composition and distribution on the outside connection surface of pleural ganglion from Aplysia with techniques of mass spectrometry

作　　者：吴韩志[1] 王磊[1] 翁鹭娜[1] 黄慧英[1] 黄河清[1] 

机构地区：[1]厦门大学生命科学学院分析测试中心厦门大学化学生物学福建省重点实验室,福建厦门361005

出　　处：《福建农林大学学报（自然科学版）》2007年第1期64-67,共4页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家自然科学基金资助项目(30470372);福建省自然科学基金资助项目(C031006);厦门大学预研基金资助项目(xdkjcx20061026;xdkjcx20051006)

摘　　要：选用基质辅助激光解吸电离化飞行时间(MALDI-TOF)质谱技术研究蓝斑背肛海兔侧膜神经节与腹部之间神经连索外表层的多肽组成和分布.采用比对法和Paws软件分析多肽序列和酶解位点,发现该神经连索表层含有丰富的酸性多肽(AP)的酶解产物,其酶解过程由AP的C到N端.表明海兔中枢神经系统外表层不仅含有丰富的多肽种类,而且还参与信号传导等调控作用.The pepfide composition and distribution on the outside surface of neural connection between pleural ganglion and abdomen were studied by matrix-assisted laser desorption ionization/time of flight （MALDI-TOF） mass spectrometry. With compared methods, paws software, abundant enzymolysis produces of acidic peptide （AP） and enzymolysis sites were found. The result suggests that the produces occur from the N to C end of AP. It is indicated that the outside connection surface of central neural system from the Aplysia has abundant peptide kinds and these peptides play an important role in the modulation of signal transportation.

关 键 词：海兔 神经连索 神经多肽 质谱 

分 类 号：Q503[生物学—生物化学]