球衣菌胞内聚β-羟基丁酸提取方法的研究  

Study on Poly-β-hydroxybutyrate(PHB)Extraction of Sphaerotilus Natans

作　　者：许旭萍[1] 陈接锋[1] 

机构地区：[1]福建师范大学生物工程学院,福州350007

出　　处：《生物技术通报》2006年第3期68-72,共5页Biotechnology Bulletin

基　　金：福建省教育厅资助项目(No.KA20097)

摘　　要：以球衣菌(Sphaerotilus natans FQ40)为材料,比较多种破壁方法对其胞内PHB提取率的影响。结果表明SDS-NaClO混合破壁法最适宜于提取球衣菌胞内PHB。细胞悬液先用10g/L SDS,35℃处理10min,再用体积分数5%的NaClO处理5min后,经离心、洗涤、烘干,用热氯仿抽提PHB,提取率可达56%,纯度与标准品相同。The effect on extraction rate of several different methods for breaking up Sphaerotilus natans cell wall was conducted. The results showed that the combined SDS-NaC10 treatment method was the optimum for extraction PFIB from S.natans.The cells was treated by 10g/L SDS at 35℃ for 10 min first, and then by 5%（v/v） NaClO for 5min. After the treated suspention being centrifuged, washed and dryed, the PFIB was extracted with hot chloroform. At this conditions, the extraction rate could reach 56%,and the purity of PHB was coincident with the standard.

关 键 词：球衣菌 PHB 提取 

分 类 号：X172[环境科学与工程—环境科学]