乙型肝炎病毒血清标志物与HBV DNA定量结果分析  

Correlation Analysis of HBV Serum Markers and HBV DNA Quantitative Detection Results

作　　者：林应标[1] 陈朝晖[2] 郭满容[1] 刘巧突[1] 刘颖慧[1] 何准发[1] 

机构地区：[1]湖南省郴州市第一人民医院检验科,湖南郴州423000 [2]中南大学湘雅医院

出　　处：《实用预防医学》2007年第1期197-198,共2页Practical Preventive Medicine

摘　　要：目的探讨乙型肝炎病毒(HBV)血清学检测出现的组合模式与HBVDNA定量之间的关系,正确评价其临床意义。方法用酶联免疫吸附试验(ELISA)检测血清标志物,用荧光定量PCR检测HBVDNA含量,依据乙肝病毒标志物阳性出现的模式分为7组A组191例,为HBsAg、HBeAg、HBcAb阳性(俗称大三阳);B组328例,为HBsAg、HBe-Ab、HBcAb阳性(俗称小三阳);C组26例,为HBsAg、HBeAg阳性;D组54例,为HBsAg、HBcAb阳性;E组35例,HBsAb、HBeAb、HBcAb阳性;F组11例,为HBsAb、HBcAb阳性;G组10例,为HBcAb阳性。结果各组HBVDNA>1.00ⅹ103拷贝/ml的阳性增高百分率分别为A组98.95%(189/191);B组46.34%(152/328);C组100%(26/26);D组57.41%(31/54);E组20.00%(7/35);F组18.18%(2/11);G组20.00%(2/10)。A组、C组与B、D、E、F、G组间HBVDNA阳性增高率有显著性差异(P<0.01),其他各组间比较有些差异有显著性,有些差异无显著性(P<0.01或P>0.05)。结论正确合理地分析HBV血清学指标出现的类型,与HBVDNA的联合动态检测有助于临床诊断、疗效观察及预后判断。Objective To explore the relationship between the different patterns of HBV markers and HBV DNA quantity, and to assess its clinical value. Methods HBV serum markers and HBV DNA quantity were detected by ELISA and fluorescence quantitative PCR respectively. According to their positive modes of HBV markers, all cases were divided into seven groups： groups A with 191 cases positive in HBsAg,HBeAg and HBcAb, groups B with 328 cases positive in HBsAg, HBeAb and HBcAb, groups C with 26 cases positive in HBsAg and HBeAg, groups D with .54 cases positive in HBsAg and HBcAb, groups E with 3.5 cases positive in HBsAb, HBeAb and HBcAb, groups F with 11 cases positive in HBsAb and HBcAb, groups G with 10 cases positive in HBcAb. Results The percentage of the positive cases （HBV DNA〉 1.00×10^3 copies/ml） in each group was 98.95 % （189/191） in group A, 46.34 % （152/328） in group B, 100% （26/26） in group C, 57.41% （31/54）in group D, 20.00%（7/35） in group E, 18.18% （2/11） in group F and 20.00% （2/10） in group G. The positive rates of HBV DNA in group A and C were significantly higher than that in group B, D, E, F and G（P〈0.01）. Conclusion Oorrelation analysis of different patterns detection of HBV serum markers and a realtime detection of HBV DNA are helpful to clinical diagnosis, treatment and monitor for the state of HBV infection and its complication.

关 键 词：乙型肝炎病毒 荧光定量PCR 定量 乙型肝炎病毒核酸 

分 类 号：R373.21[医药卫生—病原生物学]