肾癌细胞冻融抗原负载树突状细胞瘤苗的活化  

Renal tumor cell lysate antigen induces the activation of dendritic cells

作　　者：唐小龙[1] 徐萌[2] 陆大祥[3] 谢春梅[4] 蔡淑玉[4] 

机构地区：[1]暨南大学医学院血液病研究所,广东广州510632 [2]暨南大学医学院第一临床学院肿瘤科,广东广州510632 [3]暨南大学医学院病理生理教研室,广东广州510632 [4]安徽理工大学医学院生物医学工程研究所,安徽淮南232001

出　　处：《中国病理生理杂志》2007年第3期465-468,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No30070840);广东省医学科研基金资助项目(NoA2005340)

摘　　要：目的:探索体外诱导DCs活化的方法和制备肾癌树突状细胞(DCs)瘤苗。方法:制备肾癌细胞冻融抗原;取健康人新鲜血分离外周血单个核细胞(PBMC),应用GM-CSF+IL-4刺激,诱导PBMC为iDCs,然后进行分组,采用不同因子刺激iDCs转化为mDCs,其中A组:冻融抗原负载;B组:TNF-α+冻融抗原负载;C组:IL-1β+冻融抗原负载;D组:TNF-α+IL-1β+冻融抗原负载。结果:各组均可诱导iDCs的成熟,并高表达CD86、CD80和HLA-DR;相对于其它组,D组DCs更显著上调CD83和CD54表达(P<0.05)和IL-12分泌(P<0.01),且D组mDCs更有效地刺激淋巴细胞增殖(P<0.05)。结论:TNF-α+IL-1β与肾癌细胞冻融抗原协同可有效促进DCs成熟、增强诱导淋巴细胞活化的能力。AIM： To investigate the effects of renal tumor cell lysates and other cytokines on the activation of immature dendritic cells （ iDCs ）, and to develop DCs vaccines for stimulation of renal tumour - specific immunity. METHODS： DCs induced from peripheral blood mononuclear cells of healthy volunteers were cultured and propagated in vitro using rhGM - CSF and rhIL - 4, and then were cocultured with renal tumor cell lysates and different cytokines. A group ： only with renal tumor cell lysates; B group： with renal tumor cell lysates and TNF -α; C group： with renal tumor cell lysates and IL - 1β ; D group ： renal tumor cell lysates and TNF - α ＋ IL - 1β. RESULTS ： iDCs were induced to mature in all four groups, and high level expressions of CD86, CD80 and HLA - DR were observed. Compared to other groups, DCs in D group expressed CD83 and CD54 at higher level （ P 〈 0. 05 ）, secreted higher quantity of IL - 12 （ P 〈 0. 01 ）. Moreover, mDCs in D group induced multiplication of lymphopoiesis more effectively （P 〈 0. 05 ）. CONCLUSION： Renal tumor cell lysates and TNF - α ＋ IL-1β induce the mature phenotype and IL - 12 production in DCs and synergistically promote the stimulatory effects of lymphopoiesis.

关 键 词：树突细胞 肾肿瘤 抗原 肿瘤 疫苗 

分 类 号：R363[医药卫生—病理学]