重症肺炎大鼠干扰素-γ、白细胞介素-6和肿瘤坏死因子-α含量变化  

Changes of IFN-γ,IL-6 and TNF-α levels in rats with severe pneumonia

作　　者：陈业民[1] 黄文杰[1] 李胜利[1] 梁坤[1] 

机构地区：[1]广州军区广州总医院呼吸科,广东广州510010

出　　处：《中国病理生理杂志》2007年第3期492-494,共3页Chinese Journal of Pathophysiology

基　　金：广东省科技厅社会发展基金资助项目(No2005B30601011)

摘　　要：目的:研究SD大鼠细菌性重症肺炎模型中的IFN-γ、IL-6、TNF-α含量变化。方法:SD大鼠随机分为模型Ⅱ组、模型Ⅰ组和对照组,每组分为3小组,前两组每小组8只,对照组每小组4只。其中,模型Ⅱ组和模型Ⅰ组分别接种相同浓度不同剂量的肺炎克雷伯菌菌液,对照组接种剂量相同的生理盐水。分别与接种后的第2、4、6d分批处死动物,动态观察动物的干扰素-γ(IFN-γ)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)的变化情况。结果:模型Ⅱ组和模型Ⅰ组的IL-6、TNF-α于接种后第2、4、6d呈逐渐升高的趋势,尤以接种高剂量菌液模型Ⅱ组升高明显;IFN-γ呈逐渐下降的趋势,尤以接种高剂量菌液模型Ⅱ组下降明显;而模型Ⅰ组则无明显变化。结论:诸多细胞因子可能参与重症肺炎发病的病理生理过程。随着接种一定浓度菌液剂量的增加,致使大鼠肺炎明显加重,IL-6、TNF-α、IFN-γ较一般肺炎变化更显著。AIM： To study the variety of cytokines in severe bacterial pneumonia of Sprague Dawleg （SD） rats. METHODS ： A total of 60 SD rats were randomly divided into three groups ： model Ⅰ group （ n = 24 ） , model Ⅱ group （n =24）, and control group （n = 12）. Rats in the model Ⅰ group and the model Ⅱ group were intratracheally instilled with suspension of klebsieUa pneumoniae at different doses. Rats in the control group were intratracheally injected with 1 mL saline. On the 2nd, 4th and 6th day after intratracheal instillation, 1/3 rats in each group were killed to determine the concentration of IFN - γ, IL - 6 and TNF - α in blood. RESULTS ： The levels of IL - 6 and TNF - α in model groups were higher than those in control group, while the level of IFN - γ was lower. The change of cytokines was more significant in the model Ⅱ group （severe pneumonia） than those in the model Ⅰ group. CONCLUSION： The cytokines we studied may play an important role in the pathogenesis of severe pneumonia. The change of cytokines is more significant in severe pneumonia than those in common pneumonia.

关 键 词：肺炎 克雷伯菌 白细胞介素6 肿瘤坏死因子 干扰素Ⅱ型 

分 类 号：R363[医药卫生—病理学]