参附注射液对缺血再灌注肢体保护作用  

Protective effects of Shengfu injections on limbs in ischemia/reperfusion injury

作　　者：黄卫[1] 张应鹏[1] 钟波[1] 王素伟[1] 

机构地区：[1]暨南大学附属第三医院珠海市人民医院脊柱骨病科,广东珠海519000

出　　处：《中国病理生理杂志》2007年第3期540-542,共3页Chinese Journal of Pathophysiology

基　　金：珠海市医药卫生科研课题基金资助项目(NoPC20052009)

摘　　要：目的:探讨参附注射液(SF)对缺血再灌注肢体保护作用及机制。方法:30只月龄相当的健康大鼠随机分为A、B、C、D、E5组,A组为单纯缺血再灌注组(IR),B组、C组分别于阻断血流前30min股动脉注射参附注射液10mL/kg、20mL/kg;D组和E组分别于再灌注后立即股动脉注射参附注射液10mL/kg、20mL/kg。5组动物均在股动静脉阻断前后1h及再灌注后1h、2h分别从股静脉采集血样,测定血浆中肌酸磷酸激酶(CPK)、谷草转氨酶(GOT)的含量以及测定血浆超氧化物歧化酶(SOD)、丙二醛(MDA)的含量。结果:各治疗组中SOD活性明显高于IR组,MDA、CPK、GOT水平则明显低于IR组。但B组和C组之间有明显差异。而B、C组与D、E组之间有明显差异。结论:参附注射液对缺血再灌注肢体具有保护作用,但术前给药、剂量20mL/kg效果更佳。AIM： To study the protective effects of Shengfu injections on the limbs in ischemia/reperfusion injury. METHODS ： 30 healthy rats at the same age were divided into 5 groups randomly. Group A was the simple reperfusion of ischemic limbs. Group B and C were injected with the Shengfu injections through femoral arteries at doses of 10 mL/kg and 20 mL/kg. Group D and E were injected with the Shengfu injections through femoral arteries at doses of 10 mL/kg and 20 mL/kg right after reperfusions. Blood samples were collected from femoral veins 1 h before femoral vessels were blocked, 1 h and 2 h after reperfusions in all animals. The concentrations of the CPK, GOT, SOD and MDA in the blood samples were determined. RESULTS： MDA increased, SOD, CPK, GOT decreased markedly in the therapeutic groups. CONCLUSION： The Shengfu injections surely protected the limbs from ischemia/reperfusion injury, the better effects was observed when the drug was administered before operation at the dose of 20 mL/kg.

关 键 词：参附注射液 再灌注损伤 四肢 超氧化物歧化酶 丙二醛 肌酸激酶 

分 类 号：R363[医药卫生—病理学]