中国株庚型肝炎病毒C(GBV-C)感染的检测及部分核酸序列测定  

Detection of GBV-C infection and sequencing of partial gene of a chinese strain of GBV-C

作　　者：汪兴太[1] 庄辉[2] 李河民[1] 范金水[2] 祁自柏[1] 刘刚[3] 

机构地区：[1]中国药品生物制品检定所,北京100050 [2]北京医科大学微生物学系 [3]军事医学科学院毒物药物研究所

出　　处：《中华微生物学和免疫学杂志》1996年第4期263-266,共4页Chinese Journal of Microbiology and Immunology

摘　　要：国内首次采用合成肽抗原筛查高危人群血液中的庚型肝炎病毒Ｃ（ＧＢＶ－Ｃ）抗体。单采浆站供血者ＧＢＶ－Ｃ抗体阳性率为０．４４％（１１／２５００）；非甲－戊型肝炎患者ＧＢＶ－Ｃ抗体阳性率为６．６７％（２／３０）。抗体阳性血清再用ＲＴ－ＰＣＲ法检测ＧＢＶ－ＣＲＮＡ。部分扩增产物进行了核苷酸序列测定，其核苷酸序列与Ｓｉｍｏｎｓ等报道的ＧＢＶ－Ｃ序列同源性为８９．０９％至９２．４８％。该研究提示，ＧＢＶ－Ｃ可能不是非甲－戊型肝炎的主要致病因子，可能存在ＧＢＶ－Ｃ健康携带者，应尽快研制出ＧＢＶ－Ｃ感染检测试剂盒，以筛选献血员。Peptides of GBV -C were synthized and used to detect GBV -C infection . Antibodies were detected in 0.44%(11/2500 ) blood donors and in 6. 67% (2/30) Non A-E hepatitis patients. The results do not. suggest that GBV-C being the main cause of Non A -E hepatitis. GBV-C RNA was detected in antibodies positive sera. Partial Bene (NS3) of a Chinese GBV-C was sequenced and compared with the corresponding parts of GBV- C, reported by Simons. The nucleotide homology of the region was 89.09% between Chinese GBV-C (ChT2 ) and the GBV -C reported by Simons. The amino acid homology between the two GBV-C was 98. 82% The results also suggest that GBV-C may exist as healthy carriers. It is necessary to develop diagnostic reagents for detecting GBV-C infection and screening blood donors.

关 键 词：庚型肝炎病毒 核苷酸序列 

分 类 号：R512.63[医药卫生—内科学] R373.21[医药卫生—临床医学]