一株运动发酵单胞菌Zy-1快速生产乙醇  

Fast Production of Ethanol by Zymomonas mobilis (Zy-1)

作　　者：刘艳[1,2] 赵海[1] 戚天胜 唐秋琳 黄宇峰 

机构地区：[1]中国科学院成都生物研究所 [2]中国科学院研究生院北京100049 [3]成都固生科技发展有限公司

出　　处：《应用与环境生物学报》2007年第1期69-72,共4页Chinese Journal of Applied and Environmental Biology

基　　金：科技部攻关项目(2004BA411130);中国科学院"西部之光"项目;四川省科技攻关项目"细菌发酵燃料乙醇制备技术研究"资助(04NG002-002;027.X028-037)~~

摘　　要：经多次实验优化，得到运动发酵单胞菌Zy发酵葡萄糖生产乙醇较合适的条件．Zy的诱变菌株Zy-1在该条件下发酵葡萄糖生产乙醇比原始菌株更有较大优势．当葡萄糖浓度为200gL^-1时，发酵48h，乙醇浓度96．5gL^-1，残糖2．3gL^-1，发酵效率为94．42％．Zy-1发酵天然原料米粉、木薯、红薯干等，发酵时间44h，乙醇浓度达95gL^-1以上，发酵效率92％以上．发酵液用DNS法测定，还原糖约2gL^-1，残总糖因原料种类不同，其值有所差异（5～20gL^-1）．经薄层层析分析，发酵液无葡萄糖，而是二糖、三糖等低聚糖．A good condition for fermenting glucose into, ethanol by Zymomonas mobilis （Zy） was achieved. Under the condition, its mutant （ Zy-1 ） was more capable of producing ethanol than its original strain. When glucose concentration was 200 g L^-1, the ethanol concentration was 96.5 g L^-1 and stoichiometric yield was 94.42% by Zy-1 fermentation for 48 h. In addition, as Zy-1 fermented raw materials, such as rice flour, cassava flour and sweet potato into ethanol under the optimum condition, the ethanol concentration could be over 95 g L^-1 and stoichiometric yield above 92%, with fermentation time of 44 h. The sugar concentration in fermentation liquor was measured by the DNS method. The reducing sugar was about 2 g L^-1 , but residual sugars were different owing to different raw materials （from 5 g L^-1 to 20 g L^-1 ）. The analysis by thin-layer chromatogram indicated that in the fermentation liquor there was no glucose, but there were disaccharide and trisaccharide. Fig 2, Tab 5, Ref 12.

关 键 词：运动发酵单胞菌(Zymomonas mobilis) 细菌发酵 乙醇 诱变菌株 米粉 木薯 红薯干 

分 类 号：TQ223.122[化学工程—有机化工]