三种细胞辐射敏感性相关酶的作用研究  

Role of topoisomerase Ⅰ,Ⅱ and poly(ADP-ribose) polymerase in cell radiosensitivity

作　　者：罗瑛[1] 孙志贤[1] 夏寿萱 

机构地区：[1]北京放射医学研究所

出　　处：《中华放射医学与防护杂志》1996年第4期232-234,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金

摘　　要：拓朴异构酶Ⅰ和Ⅱ、聚腺苷二磷酸核糖聚合酶等是参与调节染色质结构的关键酶，在ＤＮＡ复制、转录及损伤修复等重要的生命事件中起着十分重要的作用。本实验采用正交设计方法分析它们在细胞染色质调控方面的功能以及对辐射所致ＤＮＡ损伤的影响。结果表明，经过相应的酶抑制剂作用后，染色质结构的松弛程度增加，受照后ＤＮＡ的初始损伤加重，且三种酶之间存在交互作用，提示出这些酶可能在使染色质结构紧密化及提高辐射抗性方面起有利作用；所观察到的交互作用可能反映了三种酶之间的联系。TopoisomeraseⅠ,Ⅱ and poly(ADP-ribose) polymerase are key enzymes in regulation of chromosome structure and play important roles in DNA replication,RNA transcription and DNA damage repair.We used an orthogonal design to analyze the role of topoisonoraseⅠ,Ⅱ and poly(ADP-ribose) polymerase in the regulation of chromosome structure and the production of DNA damages after irradiation.It was observed that their inhibitors could loose chromosome structure and increase DNA damages in irradiated cells,and they interacted with each other.This result shows that these three enzymes facilitate the integrity of chromosome structure and promote radioresistant ability of cells.The interaction between these three enymes reflects their internal relation.

关 键 词：细胞 酶类 辐射敏感性 拓扑异构酶 染色质结构 

分 类 号：R811.5[医药卫生—放射医学] Q345.22[医药卫生—临床医学]