胰岛素样生长因子结合蛋白-7′在大鼠胚胎植入过程中的差异表达与调节  

The Differential Expression and Regulation of IGFBP-7′ in The Process of Embryo Implantation in Rats

作　　者：夏红飞[1] 孙敬[1] 杨颖[1] 彭景楩[1] 

机构地区：[1]中国科学院动物研究所计划生育生殖生物学国家重点实验室

出　　处：《生物化学与生物物理进展》2007年第3期267-274,共8页Progress In Biochemistry and Biophysics

基　　金：国家重点基础研究发展计划(2006CB944007;2006CB504006);国家自然科学基金(30370165)资助项目~~

摘　　要：胰岛素样生长因子结合蛋白-7(IGFBP-7)已经证实在人的妊娠过程中起了重要作用,但在大鼠中尚未见报道.在过去的研究中曾利用抑制消减杂交(SSH)方法分析了植入前和植入期的基因表达谱,发现IGFBP-7存在差异表达.通过RNA印迹和原位杂交,分析了IGFBP-7部分序列(编码区531～928nt,称作IGFBP-7′)在大鼠妊娠早期子宫中的时空表达模式.用RT-PCR方法检测了其在不同组织器官及假孕、人工诱导蜕膜化和延迟着床激活大鼠子宫中的表达模式.结果显示:在大鼠妊娠第5天IGFBP-7′mRNA的表达量开始增加,第5.5和6天表达量显著高于植入前期.IGFBP-7′mRNA主要表达于子宫腔上皮和腺上皮.IGFBP-7′mRNA表达无组织特异性,在大鼠的下丘脑、垂体、卵巢、子宫、心、肝、脾、肺、肾等器官均有表达,在假孕的D1～D6大鼠子宫中均有表达,但无显著性差异,诱导蜕膜化后IGFBP-7′mRNA的表达量也无明显变化,但在延迟着床激活的大鼠子宫中表达显著增加.这些结果提示,在植入期IGFBP-7′的表达增加主要是由胚泡引起的,而非蜕膜化.在大鼠妊娠早期,IGFBP-7′的表达增加可能有利于胚胎植入的发生.IGFBP-7 plays an important role in the process of human pregnancy. However, it is not found in rats. In previous study, suppression subtractive hybridization （SSH） was utilized to analyse differentially expressed gene between pre-implantation period and implantation period and IGFBP-7 was identified as a gene differentially expressed. Temporo-spatial expression pattern of partial IGFBP-7 cDNA sequence（CDs 531-928 nt, IGFBP-7＇） in early pregnancy was detected by Northern blot and in situ hybridization. RT-PCR was used to analyse IGFBP-7＇ mR.NA expression in multiple tissue and uterus of pseudopregnancy, artificial decidualization and delayed-implantation. Results indicated that IGFBP-7＇ mRNA expression level began to increase in the day 5 ofpregnancy, the expression level during day 5.5 and 6 is higher than pre-implantation period. IGFBP-7＇ mRNA was mainly located in luminal epithelium and gland epithelium. The expression of IGFBP-7＇ mRNA have not tissue-specific and was detected in hypothalamus, pituitary, uterus, ovary, heart, liver, spleen, lung, kidney and so on. The expression of IGFBP-7＇ mRNA was not significantly different in uterus of pseudopregnancy and artificial decidualization, and significantly increased in the uterus of delayed-implantation. Collectively, these results suggest that IGFBP-7＇ expression increase is mainly induced by blastocyst, not decidualization during the window of implantation, which may be beneficial to embryo implantation in the early of pregnancy in rats.

关 键 词：胰岛素样生长因子结合蛋白-7’ SD大鼠 子宫 胚胎植入 

分 类 号：Q4[生物学—生理学]