不同龋敏感儿童牙菌斑内口腔链球菌的PCR-DGGE分析  

Analysis of oral Streptococci in dental plaque of different caries-susceptibile children by PCR-DGGE

作　　者：姜云涛[1] 梁景平[1] 李超伦[2] 杨桂梅[3] 张明珠[1] 任大明[3] 

机构地区：[1]上海交通大学医学院第九人民医院牙体牙髓科上海市口腔医学重点实验室,上海200011 [2]上海交通大学医学院第九人民医院牙周科上海市口腔医学重点实验室,上海200011 [3]复旦大学遗传学研究所遗传工程国家重点实验室,上海200433

出　　处：《上海交通大学学报（医学版）》2007年第2期133-136,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金："十五"国家科技攻关计划(2004BA720A24);国家自然科学基金(30572039)~~

摘　　要：目的 应用PCR-变性梯度凝胶电泳（DGGE）技术，分析不同龋敏感儿童牙菌斑内口腔链球菌菌群及其菌种组成的多样性。方法 牙菌斑组织取自45例学龄前儿童，根据乳牙龋失补牙面（dmfs）指数分为无龋（dmfs=0）、中龋（dmfs=4～6）和高龋（dmfs〉8）（n=15）。分别提取细菌总DNA，进行链球菌属mp B特征序列的PCR扩增及DGGE分析，克隆、测序并与核酸序列数据库的序列进行比对。结果 无龋儿童菌斑中口腔链球菌菌群的基因型分布均显著高于中龋和高龋儿童（P〈0．05）；变异链球菌群是高龋组中的优势菌群。结论 PCR—DGGE技术结合rnpB基因克隆文库分析可较灵敏、直观地反映牙菌斑中链球菌属各菌群及其菌种的组成情况。Objective To study the diversity of oral Streptococci in dental plaque of different caries-susceptibile children by PCR- denaturing gradient gel electrophoresis （DGGE）. Methods Total DNAs were extracted from the plaques of 45 pre-school children, 15 in caries-free group （ dmfs = 0） , 15 in moderate-caries group （ dmfs = 4 - 6） and another 15 in caries-susceptive group （ dmfs 〉 8）. Bacterial communities in the three groups were examined by PCR of rnp B and DGGE and by analyzing the sequences of the ribotypes. Results Significantly greater diversity in genetypes of Streptococcus oralis group was found in the caries-free group than the moderatecaries group and caries-susceptive group （P 〈0.05）. Streptococcus mutans group was the predominant flora in caries-susceptive children. Conclusion PCR-DGGE method combined with rnp B clone library profiling is a sensitive and effective approach to resolve the population structure of oral Streptococci in microflora of dental plaque.

关 键 词：牙菌斑 细菌群落 变性梯度凝胶电泳 链球菌 口腔 

分 类 号：R788.1[医药卫生—口腔医学]