远缘链球菌6715耐氟菌株与亲代菌株的基因表达差异  

Differential gene expression between Streptococcus sobrinus 6715 and its fluoride-resistant strains

作　　者：姜葳[1] 梁景平[1] 李超伦[2] 姜云涛[1] 张明珠[1] 

机构地区：[1]上海交通大学医学院第九人民医院牙体牙髓科上海市口腔医学重点实验室,上海200011 [2]上海交通大学医学院第九人民医院牙周科上海市口腔医学重点实验室,上海200011

出　　处：《上海交通大学学报（医学版）》2007年第2期137-140,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金："十五"国家科技攻关计划(2004BA720A24)~~

摘　　要：目的比较远缘链球菌6715耐氟菌株与亲代菌株的基因表达差异。方法采用逐步诱导法诱导远缘链球菌6715产生耐氟突变株,分别提取耐氟突变株与亲代菌株菌细胞总RNA并分离纯化mRNA,经逆转录获得cDNA后进行抑制性消减杂交(SSH),比较分析两者的基因表达谱;差异片段通过克隆测序并经在线同源性分析(BLAST)获得片段的基因信息。结果经与GenBank的BLAST比对,确证存在两对差异片段,分属结构基因fruA和SMU.438 c。结论应用SSH技术成功构建远缘链球菌6715耐氟菌株与亲代菌株基因差异表达的cDNA消减文库,为深入研究其耐氟机制奠定了基础。Objective To detect the differential gene expression between Streptococcus sobrinus（S, sobrinus） 6715 and its fluoride-resistant strains. Methods The fluoride-resistant strains of S. sobrinus 6715 was induced by increasing the concentration of fluoride step by step. Total RNA of both S. sobrinus 6715 and its fluoride-resistant strains was extracted, mRNA was separated and purificated, and then cDNA was obtained by reversed transcription. Suppression subtractive hybridization （SSH） technology was used to detect the differential gene expression between them. The differential gene expression fragments were cloned and compared with the GenBank by BLAST. Results After comparing with the GenBank by BLAST, it was identified that there were two differential gene expression fragments, fruA and SMU. 438c. Conclusion The cDNA subtractive lib of differential gene expression between S. sobrinus 6715 and its fluorideresistant strains was successfully constructed through SSH, which paves a way for the further study of fluoride-resistant mechanism.

关 键 词：远缘链球菌 耐氟菌株 抑制性消减杂交 基因表达 

分 类 号：R780.2[医药卫生—口腔医学]