纳米粒子载体胶质细胞源性神经营养因子在胶质瘤细胞中的表达与作用实验研究  

Experimental study of expression and effects of the use of chiston nanoparticle vector and glial cell derived neurotrophy factor in gliomas cells

作　　者：刘钦毅[1] 杨有庚[1] 赵红光[2] 刘赤兵[1] 

机构地区：[1]吉林大学第二医院骨科,吉林长春130041 [2]吉林大学公共卫生学院,吉林长春130021

出　　处：《中国实验诊断学》2007年第3期355-357,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的观察壳聚糖纳米粒子载体载基因在胶质瘤细胞中表达及其对神经细胞的保护作用。方法采用免疫细胞化学检测胶质细胞源性神经营养因子(GDNF)在胶质细胞瘤细胞中的表达,采用细胞增殖实验(MTT法)观察GDNF对神经细胞的营养作用。结果GDNF在胶质细胞瘤细胞中有表达,在应用纳米粒子载体载基因进入细胞内后GDNF得到高效表达,并保护神经细胞,促进细胞增殖。结论应用纳米粒子载体载基因进入细胞内后可有效提高GDNF表达,并保护神经细胞,促进细胞增殖。对脊髓损伤后应用这一方法治疗提供理论依据。Objective To study the expression of chitason nanoparticle vector and GDNF gene in gliomas cells and its protective function.Methods To examine the expression of GDNF in gliomas celLs by the cyto- immunochemistry and to study the nutrition effect. Results GDNF has been expressed in gliomas cells and been highly expressed by the use of chitosan nanoparticle gene complex. The high expression of GDNF can protect neuron and promote proliferation of the cell. Condusion The use of chitosan nanopartide gene complex can highly express GDNF and protect neuron cell. It can provide theory basis for its use in Spinal Cord Injury.

关 键 词：纳米粒子 壳聚糖 胶质细胞瘤细胞 胶质细胞源性神经生长因子 

分 类 号：R739.41[医药卫生—肿瘤]