碱性成纤维细胞生长因子在酒精性肝病大鼠中的表达及抗纤复方I号对其影响  

Effect of Kangxian Fufang Ⅰ on expression of basic fibroblast growth factor in rats with alcoholic liver disease

作　　者：崔巍[1] 敖然[1] 傅宝玉[1] 

机构地区：[1]中国医科大学附属第一医院消化内科,辽宁沈阳110001

出　　处：《中国医科大学学报》2007年第1期59-61,共3页Journal of China Medical University

摘　　要：目的：探讨碱性成纤维细胞生长因子（BFGF）在酒精性肝病大鼠肝脏中的表达及抗纤复方I号对其表达的影响。方法：Wistar大鼠给予乙醇8～10g·kg^-1·^-1，3次灌胃12周制备酒精性肝病大鼠模型为酒精组，对照组给予等量生理盐水，中药组在给予同酒精组等量乙醇的基础上，给予抗纤复方I号水煎剂4．0mL·ks^-1·d^-1，2次灌胃，共12周。实验4、8、12周末分批处死动物，HE及VG染色观察肝脏组织学改变。应用免疫组织化学技术检测BFGF在酒精性肝病过程中的定位及表达，并用病理图象分析仪对其结果进行半定量分析。结果：造模12周时，酒精组大鼠肝脏呈现重度脂肪变性，点片状肝细胞坏死及炎性细胞浸润，纤维条索形成；而中药组大鼠仅呈现轻度脂肪变，无纤维条索形成。BFGF表达在正常对照组主要位于血管内皮细胞的胞浆中，中药组表达同正常对照组基本一致；在酒精组BFGF的表达除位于血管内皮细胞外，还位于窦周细胞、纤维间隔内浸润细胞的胞浆及汇管区、纤维间隔区的细胞外基质中。结论：在酒精性肝病BFGF的表达增强，抗纤复方I号能降低其表达。Objective： To investigate the expression of basic fibroblast growth factor （BFGF） in rats with alcoholic fiver disease and the effect of Kang：dan Fufang I （KXI） on BFGF expression. Methods：Wistar rats weighing 160 to 200 g were lavaged with normal saline （6.0 ml·kg^-1·d^-1,ethanol （8 to 10 g·kg^-1·d^-1,and ethanol plus KXI （4.0 ml·kg^-1·d^-1） for 12 weeks in control group,alcohol group,and traditional Chinese medicine group,respectlvely. At the end of 4,8,12 weeks,the rats were kiUed,and the histopathological changes of the fiver were observed The BFGF expression in the fiver was examined by immtmohistochemical method. Results：Slight steatosis was observed in alcohol group at week 4. At week 12,marked hepatic fatty changes,inflammation,necrosis and mild fibrosis were also observed. In conwast, these alterations were attenuated by KXI administration,although mild fatty changes remained. In normal liver,BFGF expressed in endothelial cells lining major vessels whereas positive reactions were observed in sinusoidal cells and extracelltdar matrix of fibrous bands in ethanol-fed rats. Conclusion：In rats with alcoholic fiver disease,BFGF is highly expressed,and KXI could decrease BFGF expression.

关 键 词：酒精性肝病 大鼠 中药 碱性成纤维细胞生长因子 

分 类 号：R573[医药卫生—消化系统]